从冻藏凝血块中制备DNA技术  

DNA Preparation from Frozen BloodClots

作　　者：赵庆伟[1] 申萍 周梦玲[1] 张华[1] 余铭鹏[1] 

机构地区：[1]中国协和医科大学基础医学院病理学教研室,北京100005

出　　处：《中国动脉硬化杂志》1998年第3期257-258,共2页Chinese Journal of Arteriosclerosis

基　　金：国家"攀登计划"

摘　　要：从凝血块中分离DNA技术在现有分子生物学实验技术参考书中没有具体的叙述。本文归纳出一套自冻藏凝血块中制备高分子量基因组DNA技术，经长期应用表明该方法简便可靠．易于掌握．可快速地进行大量标本的DNA制备；尤其适用于流行病学现场调查采集的血液标本的处理。In order to extract genomic DNA from frozen blood clots,a new exclusive method was esteblished,Long time application of it indicates that this method is easy, stable and expeditious, About 30 μg DNA is obtained from 1 cm3 clo,It is very suitable to pre-pare genomic DNA from large samples of blood gotten in epidemic fields survey,

关 键 词：冻藏凝血块 制备 DNA 

分 类 号：R446.8[医药卫生—诊断学]