RNA干扰技术在裸鼠移植瘤中的应用  

Application of RNA interference in xenografted tumor of nude mice in vivo

作　　者：王敬春(综述)[1] 王惠民(审校)[1] 

机构地区：[1]南通大学附属医院检验医学中心,江苏南通226001

出　　处：《肿瘤研究与临床》2009年第12期862-864,共3页Cancer Research and Clinic

基　　金：基金项目：江苏省医学重点学科建设资助项目（XK200723）

摘　　要：RNA干扰（RNAi）技术是由人工导入的双链RNA介导的特异性的转录后基因沉默技术。近年RNAi技术已大量应用于裸鼠移植瘤中，RNAi裸鼠体内实验，通过敲低肿瘤的凋亡抑制基因、促增生基因、肿瘤细胞浸润转移基因的表达，达到抑制肿瘤的效果。同时，RNAi在裸鼠移植瘤内可作为肿瘤放化疗的辅助手段。RNA interference （RNAi） is a post-transcriptional suppression method of introduction of double-stranded RNA molecules by artificially induced. Recently RNAi has been widely used in xenografted tumors of nude mice in vivo. In order to inhibit the growth of tumor, some genes related with tumor such as anti-apoptosis gene, proliferation promoting gene, metastasis-associated gene are depressed via RNAi. Additionally, in the nude mice test, RNAi is utilized as assisting methed of radiotherapy and chemotherapy of human tumors.

关 键 词：RNA干扰 基因 肿瘤治疗方案 

分 类 号：R512.62[医药卫生—内科学] R739.8[医药卫生—临床医学]