羊泰勒虫病血清学调查  

A serological survey of ovine Theileriosis by ELISA

作　　者：马米玲[1] 罗建勋[1] 关贵全[1] 刘志杰[1] 李有全[1] 高金亮[1] 刘爱红[1] 刘军龙[1] 任巧云[1] 殷宏[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室,甘肃兰州730046

出　　处：《中国兽医学报》2009年第12期1575-1577,1581,共4页Chinese Journal of Veterinary Science

基　　金：国家"863"基金资助项目(2006AA10A207);国家支撑计划基金资助项目(2007BAD40B06);国家自然资源平台资助项目(2005DKA211000)

摘　　要：应用酶联免疫吸附试验(ELISA)对我国部分省(区)的羊泰勒虫病进行了血清学调查,以便了解近年来我国羊泰勒虫病的流行现状。2004-2005年间,在我国部分省(区)总共采集到1 170份绵羊和山羊血清,并用间接ELISA检测抗羊泰勒虫抗体。结果显示,在5省13个县中除甘肃景泰县无羊泰勒虫病,其他地方均有羊泰勒虫病,且甘肃临洮感染率最高为93.8%,表明羊泰勒虫病在我国北方羊群中流行仍相当严重。此外,从2001年4月至2002年3月,从野外采集青海血蜱感染3只绵羊,收集1年的血清,采用ELISA方法,对羊泰勒虫血清抗体水平动态进行了评定,结果表明,实验室人工感染的抗羊泰勒虫抗体水平在感染后40 d最高,按此结果推算,在野外蜱的出现时间是4月份,收集血清应在5月中旬。A serological surveillance was carried out using an enzyme-linked immunosorbent assay （ELISA） for investigating the situation of ovine Theileriosis in China. During 2004 to 2005 ,the total 1 170 sera samples of sheep and goats were collected from some provinces in China, which include 13 counties in 5 provinces, and detected the anti theileria antibody by the ELISA. The results of detection indicated that ovine Theileriosis had prevalent in 13 counties in 5 provinces but not Jingtai county, the highest naturally infested positive rate of theileria was 93.8 % in Lintao, which suggested that ovine Theileriosis was seriously in China. In addition, the sera were collected from three sheep experimentally infected ovine Theileriosis by Haernaphyslia qinghaiensis in the field during April 2001 to March 2002, were used to evaluate the annual kinetics of average values of anti-theileria antibody. The kinetics showed that anti-theileri antibody kept in higher level in 40 d after infestation. It is concluded that the optimal time for sera collection is mid-May when the ticks emerged in early of April in the field.

关 键 词：血清学调查 羊泰勒虫病 ELISA 

分 类 号：S852.72[农业科学—基础兽医学]