新型细胞固定化载体强化白腐菌降解活性染料  

Enhanced biodegradation of reactive dye by immobilized white rot fungi Phanerochaete chrysosporium

作　　者：高大文[1,2] 梁红[2] 曾永刚[2] 

机构地区：[1]哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨150090 [2]东北林业大学林学院,哈尔滨150040

出　　处：《哈尔滨工业大学学报》2009年第10期52-57,共6页Journal of Harbin Institute of Technology

基　　金：国家自然科学基金资助项目(20847001);教育部新世纪优秀人才资助计划项目(NCET-05-0330);全国优秀博士学位论文作者专项基金资助项目(200544);黑龙江省自然科学基金资助项目(E200834)

摘　　要：为提高白腐真菌的产酶能力和后续对染料的降解效果,采用摇瓶试验研究新型细胞固定化载体——聚氨酯泡沫固定化白腐真菌后的锰过氧化物酶(MnP)产生情况以及后续对活性染料——活性艳红K-2BP的降解效果.结果显示,新型载体固定化培养白腐真菌的5d脱色率为95%,比悬浮培养7d的脱色率高15%.固定化培养产生的MnP酶活为936.61U/L,而悬浮培养的MnP仅为269.52U/L,并且酶活高峰期提前4d.另外,固定化培养体系中碳、氮源的消耗比悬浮培养快很多,而且固定化培养使得白腐真菌具有较高的H2O2产量.因此,新型细胞固定化载体强化白腐真菌降解活性染料的原因应归于这种培养体系碳、氮源的快速消耗而带来的MnP酶活的提高和高H2O2产量.In order to enhance enzyme production and degradability of dyestuff, white rot fungus Phanerochaete chrysosporium immobilized with polyurethane foam carriers was investigated in agitated Edenmeyer flasks. Results showed that the decolorization of 95 % was achieved in the immobilized system only after incubating Phanerochaete chrysosporium for 5 d, which was higher than 15 % in the free suspended culture for 7 d. The maximum activity of MnP was 963.61 U/L in the immobilized culture, while it was 269.52 U/L in the suspended culture. Moreover, the consumption of carbon and nitrogen substrates in the immobilized culture was much faster than that in the free suspended culture, and the production of H2O2 in immobilized culture was higher than that in the free suspended culture. It can be concluded that the enhanced biodecolorization of reactive dyes in immobilized culture of Phanerochaete chrysosporium is due to the increased activity of MnP and H2O2 concentration caused by fast consumption of carbon and nitrogen substrates in the culture system.

关 键 词：白腐真菌 PHANEROCHAETE CHRYSOSPORIUM 生物脱色 固定化培养 MNP 

分 类 号：X703[环境科学与工程—环境工程]