银杏叶提取物对痴呆大鼠学习记忆能力的干预及机制研究  

Effects of ginkgo biloba extract on spatial learning and memory in dementia rats

作　　者：吴禹[1] 李小凤[1] 余震[1] 陈莉芬[1] 谢运兰[1] 胡长林[1] 

机构地区：[1]重庆医科大学附属第二医院神经内科,重庆400010

出　　处：《激光杂志》2009年第6期82-83,共2页Laser Journal

基　　金：重庆市卫生局科研基金项目(编号:07-2-38)

摘　　要：目的:探讨银杏叶提取物对痴呆大鼠学习记忆能力的影响。方法:建立痴呆动物模型。40天后通过明暗箱避暗回避实验测试其学习记忆能力和测定脑组织匀浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力、丙二醛(MDA)含量和乙酰胆碱酯酶(AchE)活力,同时观察海马CAl区病理组织学变化。结果:与空白组比较,模型组大鼠避暗实验错误次数增多,潜伏期延长,大鼠脑组织的SOD、GSH-Px活力降低,AchE活力和MDA含量增高,其差异有显著性。与模型组比较,治疗组大鼠避暗实验错误次数减少,潜伏期缩短,大鼠脑组织的SOD、GSH-Px活力增高,AchE活力和MDA含量降低,脑组织病理改变较模型组轻,其差异有显著性。结论:银杏叶提取物可增强脑组织SOD、GSH-Px活力,降低AchE活力和MDA含量,清除氧自由基,抑制神经细胞凋亡,改善痴呆大鼠学习记忆能力。Objective：To investigate effects of ginkgo biloba extract（GbE）on spatial learning and memory in dementia rats.Methods：Dementia rat models were established.Normal group and model group were administered normal saline,GbE group was administered ginkgo biloba extract.The superoxide dismutase（SOD）,glutathion peroxidase（GSH-Px） activities,acetylcholinesterase （AchE） activities and the malondiadehyde（MDA） content in the cerebral tissue were detected till 40days.Meanwhile the neurons of the hippocampal CA1 was observed.Results：The SOD and GSH-Px,in the GbE group increased,while the MDA content and AchE activities decreased significantly compared with the model group P0.05.The results indicated that the pathological changes of the GbE group were slighter than the model group.Conclusion：The results suggest that the administration of ginkgo biloba extract on dementia rats could increase the SOD and GSH-Px,decrease the MDA content,and AchE activities,then protecting nerve cell from oxidizing injury.

关 键 词：银杏叶提取物 痴呆 大鼠 学习记忆 

分 类 号：R477[医药卫生—护理学]