猪传染性胸膜肺炎放线杆菌分离株的分子生物学鉴定  

Identification of Porcine Actinobacillus pleuropneumonia Isolates

作　　者：苗立中[1] 王艳[1] 吴忆春[2] 李峰[1] 林初文[1] 沈志强[1] 

机构地区：[1]山东省滨州畜牧兽医研究院,山东滨州256600 [2]山东省滨州职业学院生物工程系,山东滨州256603

出　　处：《动物医学进展》2009年第12期30-33,共4页Progress In Veterinary Medicine

摘　　要：根据已有的猪传染性胸膜肺炎放线杆菌(APP)dsbE基因序列,合成了一对特异性引物,从3株APP分离菌(BZ1株,BZ2株和BZ3株)均扩增出预期342bp的DNA片段。分别将扩增的目的基因连接到pMD18-T载体中,构建重组质粒,转化大肠埃希菌DH5α感受态细胞,提取质粒并进行序列分析。结果表明BZ1株dsbE基因与所有14种血清型APP参考株dsbE基因的同源性达98%～100%,BZ2株dsbE基因与所有14种血清型APP参考株dsbE基因的同源性达98%～99%,BZ3株dsbE基因与所有14种血清型APP参考株dsbE基因同源性达97%～98%,证明3株分离菌均为APP。According to disulfide bond formation protein E （dsbE） gene sequence of the Actionobacillus pleuropneumoniae （APP）, a pair of specific primers were designed, and the target fragment of 349, bp from three App strains （BZ1, BZ2, BZ3 strains） was obtained respectively by PCR. The three products were ligated with pMD18- T Vector respectively, and then transformed into bacteria DH5a competent cells for re- combinant plasmid extraction,sequence and analysis. Compared to reference strains dsbE gene of the APP all 14 serotypes, BZ1, BZ2 and BZ3 strains of nucleotide homologies were 98%-100%, 98%-99% and 97 %-98 %, respectively. The results confirmed that the three isolates are Actinobacillus pleuropneumonia.

关 键 词：传染性胸膜肺炎放线杆菌 dsbE类基因 克隆 序列分析 鉴定 

分 类 号：S852.619[农业科学—基础兽医学]