咪达唑仑对布比卡因致惊厥作用的影响  

Influences of midazolam on bupivacaine-induced convulsion

作　　者：申璐[1,2,3] 王贺[1,2,3] 赵娟[1,2,3] 赵志龙[1,2,3] 戴体俊[1,2,3] 

机构地区：[1]徐州医学院公共卫生学院 [2]徐州医学院麻醉学院 [3]徐州医学院麻醉药理学教研室,徐州221002

出　　处：《药学与临床研究》2009年第6期452-454,共3页Pharmaceutical and Clinical Research

基　　金：国家自然科学基金资助项目(30872432;30471657;39970715);江苏省自然科学基金资助项目(BK200143)

摘　　要：目的:观察咪达唑仑(midazolam)对布比卡因(bupivacaine)致惊厥作用及半数有效量(ED50)、半数致死量(LD50)的影响。方法:皮下注射咪达唑仑20min后,腹腔注射不同剂量布比卡因,观察小鼠惊厥的潜伏期、持续时间、发作等级、未惊厥数和死亡情况,并以序贯法测定预先给咪达唑仑20min后布比卡因的致惊厥ED50和LD50。结果:0.25mg.kg-1咪达唑仑可延长布比卡因致惊厥潜伏期(P<0.05,P<0.01),0.5、1.0mg.kg-1咪达唑仑可缩短惊厥持续期(P<0.01),减少惊厥发生率和致死率,降低发作等级;且本实验剂量咪达唑仑(0.25、0.5、1.0mg.kg-1与1.0、2.0、4.0mg.kg-1)均可增大布比卡因致惊厥ED50和LD50。结论:咪达唑仑可拮抗布比卡因的致惊厥作用,减轻布比卡因的毒性。Objective： To observe the influences of midazolam on bupivacaine-induced convulsion. Methods： Bupivacaine was administered intraperitoneally twenty minutes after midazolam had been administered subcutaneously to observe the convulsant latency period, convulsion duration, the level of convulsion, incidence of no convulsion and mortality, and analyze the LD50 and the ED50 by sequential method in mice. Results： The convulsant latency periods of bupivacaine- induced were extended by midazolam, the convulsion durations were shorten, the level of convulsion were cut down, and the incidence of convulsion and mortality were reduced. The ED50 of bupivacaine-induced convulsion were raised by midazolam（0.25, 0.5, 1.0 mg·kg^-1 ） ; the LD50 were raised by midazolam （ 1.0, 2.0, 4.0 mg·kg^-1）. Conclusions： Midazolam can antagonize the bupivacaine-induced convulsion.

关 键 词：咪达唑仑 布比卡因 惊厥 半数有效量 半数致死量 

分 类 号：R965.3[医药卫生—药理学] R969.2[医药卫生—药学]