过敏性紫癜患儿血循环内皮细胞检测的意义  

Significance of Determination of Circulating Endothelial Cells in Children with Henoch-Schonlein Purpura

作　　者：郭喜霞[1] 王华[1] 张建江[1] 郑莉萍[1] 

机构地区：[1]郑州大学第一附属医院儿内科,郑州450052

出　　处：《实用儿科临床杂志》2009年第21期1654-1655,1658,共3页Journal of Applied Clinical Pediatrics

基　　金：河南省医学科技攻关项目资助(200803038)

摘　　要：目的检测过敏性紫癜(HSP)患儿循环内皮细胞(CEC)水平,探讨其在HSP发病机制中的作用及其与HSP的疗效和过敏性紫癜性肾炎(HSPN)的关系。方法选择2008年5-12月在郑州大学第一附属医院儿内科住院的HSP患儿66例为研究组,本院儿科门诊健康体检儿童30例为健康对照组,患儿及健康对照组儿童均于清晨抽取空腹静脉血1mL,应用流式细胞术检测其CEC水平,比较急性期HSP患儿与健康对照组儿童之间的差异,HSP患儿肾脏受累组与无肾脏受累组之间的差异及HSP患儿缓解期肾脏受累组与无肾脏受累组之间的差异。应用SPSS13.0软件进行统计学处理。结果HSP患儿急性期CEC数量[(47.9345±16.9026)%]较健康对照组[(37.4367±10.2007)%]显著升高(t=2.900P<0.05)。伴肾受累组CEC数量[(50.8027±15.5269)%]与无肾受累组[(47.9345±16.9026)%]间比较差异无统计学意义(t=0.716P>0.05)。HSP患儿缓解期肾脏受累组CEC数量[(50.2588±12.8242)%]较缓解期无肾脏受累组[(40.8640±8.1652)%]显著升高(t=2.906P<0.05)。结论血管内皮细胞损伤在HSP的发病机制中起重要作用,CEC可用于判定HSP患儿缓解期是否并肾脏受累,且其对判定HSP患儿疗效有一定的预示作用。Objective To explore the importance effect of circulating endothelial cells （CEC） in the pathogenesis of Henoch - Schonleiu purpura （HSP） by determining the level of CEC in children with HSP, and to explore the relationship between CEC and therapeutic effect of HSP and the relationship between CEC and Henoeh - Sehonlein purpura nephritis （HSPN）. Methods Sixty - six inpatients with HSP and 30 children as healthy control group were selected in the First Affiliated Hospital of Zhengzhou University from May to Dee. 2008. The blood of all children, including the children in healthy cbntrol group, were sampled 1 mL in the early morning. And the CEC of all children were measured by flow cytometry method. The variation of CEC was analyzed in patients with HSP at acute stage and in healthy control group, in patients with HSP and in patients with HSPN, and in patients with HSP without renal damage at recovery stage and in patients with HSPN at recovery stage. SPSS 13.0 statistical software was used to analyze the data. Results CEC in patients with HSP at acute stage [ （ 47. 934 5 ± 16.902 6） % ] was significantly higher than that in healthy control group[ （37. 436 7 ± 10. 200 7 ） % ] （ t = 2. 900 P 〈 0.05 ）. CEC in patients with HSPN [ （50. 802 7 ± 15. 526 9 ） % ] was higher than that in patients with HSP[ （47. 934 5 ± 16. 902 6 ） % ] （ t = 0. 716 P 〉 0.05）. CEC in patients with HSPN at recovery stage[ （50. 258 8 ± 12. 824 2） % ] was significantly higher than that in patients with HSP without renal damage at recovery stage [ （40. 864 0 ± 8. 165 2） % ] （ t = 2. 906 P 〈 0.05）. Conclusions The damage of vascular endothelium plays an important role in the pathogenesis of HSP. The levels of CEC may be used as indexes of the kidney to be involved in patients with HSP at recovery stage. The levels of CEC may be used as indexes of the therapeutic effect in patients with HSP.

关 键 词：过敏性紫癜 循环内皮细胞 内皮损伤 

分 类 号：R725.5[医药卫生—儿科]