Expression and responses to dehydration and salinity stresses of V-PPase gene members in wheat  

作　　者：Yuezhi Wang Haibin Xu Guangxiang Zhang Huilan Zhu Lixia Zhang Zhengzhi Zhang Caiqin Zhang Zhengqiang Ma 

机构地区：[1]The Applied Plant Genomics Laboratory, Crop Genomics and Bioinformatics Center & National Key Lab of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China

出　　处：《Journal of Genetics and Genomics》2009年第12期711-720,共10页遗传学报（英文版）

基　　金：supported by Outstanding Youth Fund of NNSFC (30025030);2000's Trans-Century Talent Development Program of Ministry of Education, China;GCP Project SP2-1;Transgenic program 2008ZX08009-002;‘111’ Project B08025

摘　　要：Vacuolar H^＋-translocating pyrophosphatase （V-PPase） is a key enzyme related to plant growth as well as abiotic stress tolerance. In this work, wheat V-PPase genes TaVP1, TaVP2 and TaVP3 were identified. TaVP1 and TaVP2 are more similar to each other than to TaVP3. Their deduced polypeptide sequences preserve the topological structure and essential residues of V-PPases. Phylogenetic studies suggested that monocot plants, at least monocot grasses, have three VP paralogs. TaVP3 transcripts were only detected in developing seeds, and no TaVP2 transcripts were found in germinating seeds. TaVP2 was mainly expressed in shoot tissues and down-regulated in leaves under dehydration. Its expression was up-regulated in roots under high salinity. TaVP1 was relatively more ubiquitously and evenly expressed than TaVP2. Its expression level in roots was highest among the tissues examined, and was inducible by salinity stress. These results indicated that the V-PPase gene paralogs in wheat are differentially regulated spatially and in response to dehydration and salinity stresses.Vacuolar H^＋-translocating pyrophosphatase （V-PPase） is a key enzyme related to plant growth as well as abiotic stress tolerance. In this work, wheat V-PPase genes TaVP1, TaVP2 and TaVP3 were identified. TaVP1 and TaVP2 are more similar to each other than to TaVP3. Their deduced polypeptide sequences preserve the topological structure and essential residues of V-PPases. Phylogenetic studies suggested that monocot plants, at least monocot grasses, have three VP paralogs. TaVP3 transcripts were only detected in developing seeds, and no TaVP2 transcripts were found in germinating seeds. TaVP2 was mainly expressed in shoot tissues and down-regulated in leaves under dehydration. Its expression was up-regulated in roots under high salinity. TaVP1 was relatively more ubiquitously and evenly expressed than TaVP2. Its expression level in roots was highest among the tissues examined, and was inducible by salinity stress. These results indicated that the V-PPase gene paralogs in wheat are differentially regulated spatially and in response to dehydration and salinity stresses.

关 键 词：vacuolar H^＋-pyrophosphatase abiotic stress phylogenetic analysis 

分 类 号：Q949.71[生物学—植物学] X503.231[环境科学与工程—环境工程]