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作 者:陈灵鸷[1] 杨春华[1] 张文君[1] 黄慧君[1] 唐智松[1]
机构地区:[1]四川农业大学动物科技学院草业科学系,四川雅安625014
出 处:《中国草地学报》2009年第6期59-63,共5页Chinese Journal of Grassland
基 金:四川省国际合作项目(2009HH0019);现代农业产业技术体系建设专项资金共同资助
摘 要:采用离体萌发法测定扁穗牛鞭草花粉生活力及不同保存条件下花粉寿命,运用联苯胺一过氧化氢法对大棚和大田开花植株柱头可授性进行研究。结果表明:扁穗牛鞭草花粉离体萌发率为65.67%;室温(27℃)下,干燥保存的花粉寿命为3h,湿润保存的花粉寿命为4h;外界环境因子对扁穗牛鞭草柱头可授性影响较大,大棚(平均温度27℃,平均相对湿度65%)、大田(平均温度25℃,平均相对湿度75.9%)两条件下柱头可授性随时间变化差异性显著(P〈0.01);大棚内扁穗牛鞭草植株柱头可授期为花后1~5d,大田条件下柱头可授期为花后1~3d。The objective of the research was to test pollen viability and life-span of Hemarthria compressa under different condition. Pollen viability was checked by pollen germination in vitro, and stigma recep:tivity was estimated by Benzidine-H2O2. The results showed that the pollen germination rate in vitro was 65.67%. When temperature was 27℃ , the life span under dry and wet condition Were 3 and 4 hours, respectively. Environmental factors were related to the stigma receptivity of Hemarthria compressa, there was significanl difference (P〈0.01) in plant stigma receptivity between in greenhouse (average tempera ture was 27℃ , average relative humidity was 65%) and in field (average temperature was 25℃, average relative humidity was 75. 9%) conditions, The stigma receptivity was 1 - 5 days and 1 - 3 days after blooming in the greenhouse and in the field, respectively.
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