新品系Dy-2006苜蓿同工酶酶谱表征及与其它苜蓿品种的差异  

Zymogram Phenotypes of Isozymes of New Alfalfa Strain Dy-2006 and Its Differences from Other Cultivars

作　　者：李景欣[1] 崔国文[1] 陈雅君[2] 李钢[3] 于辉[1] 殷秀杰[1] 王明君[1] 

机构地区：[1]东北农业大学动物科学与技术学院,黑龙江哈尔滨150030 [2]东北农业大学园艺学院,黑龙江哈尔滨150030 [3]黑龙江省绿色食品发展中心,黑龙江哈尔滨150090

出　　处：《中国草地学报》2009年第6期105-108,共4页Chinese Journal of Grassland

基　　金：黑龙江省自然科学基金(C200810);"十一五"国家科技支撑子项目(2008BABD3B09-03);东北农业大学科学研究基金

摘　　要：对新品系Dy-2006、龙牧801、中苜1号和肇东苜蓿的酯酶(EST)、过氧化物酶(POD)酶谱表征进行了分析。酯酶分析共检测到9条酶带,新品系Dy-2006检测到4条酶带,2条特征谱带,其它3个苜蓿品种分别检测到6条、5条和4条酶带,特征谱带依次为1条、1条和0条;过氧化物酶分析共检测到13条酶带,Dy-2006共检测到6条酶带,3条特征谱带,其余3个苜蓿品种检测到的酶带依次为7条、4条和4条,特征谱带均为1条。新品系Dy-2006与其它3个苜蓿品种存在明显的遗传差异。The zymogram phenotypes of esterase（EST） and peroxide （POD） of new alfalfa strain Dy- 2006, Longmu801, Zhongmu No. 1 and Zhaodong were analyzed. In EST analysis, 9 enzyme bands were detected; in new strain 4 enzyme bands and 2 characteristic bands were detected, and in other 3 cultivars the enzyme bands were 6, 5 and 4 separately, and the characteristic bands were 1, 1, and 0 separately. In POD analysis, 13 enzyme bands were detected; in new strain 6 enzyme bands and 3 characteristic bands were detected, and in other 3 cultivars the enzyme bands were 7, 4 and 4 separately, and the characteristic bands were 1, 1, and 1 separately. There were genetic difference between new varieties Dy-2006 and other 3 cultivars.

关 键 词：Dy-2006 紫化计蓿 同工酶 酶谱表征 

分 类 号：S541[农业科学—作物学]