Proteomics Analysis of Component in Serum with Anti-asthma Activity Derived from Rats Treated by Acupuncture  

作　　者：王宇 崔建美 马淑兰 刘艳艳 尹磊淼 杨永清 崔学军 

机构地区：[1]Laboratory of Molecular Biology (Acupuncture), Shanghai Research Institute of Acupuncture and Meridian [2]Department of Traditional Chinese Medicine, North China Coal Medical School [3]Shanghai Medical College of Fudan University

出　　处：《Journal of Acupuncture and Tuina Science》2009年第6期326-331,共6页针灸推拿医学（英文版）

基　　金：National Basic Research Program of China (973 Program, 2009CB52290);National Natural Science Funds (30873299,30701123);Shanghai Leading Academic Discipline Project (S30304)

摘　　要：Objective： To identify the differential proteins in the serum with anti-asthma activity of acupuncture to pinpoint the substance basis of anti-asthma activity of acupuncture by proteomics method. Methods： Two-dimensional electrophoresis of protein was employed to analyze the differential proteins in the serum with anti-asthma activity of acupuncture, and identify the differential proteins with mass spectrum technique. Results： In adrenaleetomy （ADX）-asthma acupuncture group and asthma acupuncture group, two-dimensional eleetrophoresis showed that there was an obvious fuscous point （MW=44.1kD, PI=5.1）, which was 60S ribosomal protein L13, or hypothetical protein FLJ32191 analyzed by mass spectrum technique, different from other groups. In ADX-asthma acupuncture group, there were two obviously fuseous points （MW=39.8kD, PI=4.9 and MW=39.5kD, PI=5.0）, which were zinc finger protein 208, and thyroid hormone receptor-associated protein analyzed by mass spectrum technique, and two special proteins （MW=17.3kD, PI〈4.5 and 16.9kD, PI〈4.5）, which were Cyclophilin A and zinc finger protein 91 analyzed by mass spectrum technique, different from other groups. Conclusion： There are multi-target effects regulating the whole body in acupuncture treating asthma relating to immunoloregulation, gene expression, and protein svnthesization. The effects of acupuncture on the resnonse nroteins need further research.目的:通过蛋白质组技术鉴定针刺抗哮喘血清中差异蛋白,明确针刺抗哮喘效应的物质基础。方法:采用蛋白质双向电泳技术,对针刺抗哮喘血清进行蛋白质组份差异分析,并对差异蛋白质点进行质谱鉴定。结果:双向电泳显示,与其它组相比,肾上腺切除哮喘模型针刺组血清与哮喘模型针刺组血清有一个明显深染的蛋白点(MV=44.1kD,PI=5.1),质谱结果为60S核糖体蛋白L13或假想蛋白FLJ32191;双向电泳显示,与其它组相比,肾上腺切除哮喘模型针刺组血清有两个高峰度蛋白点(MW=39.8kD,PI=4.9和MW=39.5kD,PI=5.0),质谱分析表明依次为锌指蛋白208、甲状腺激素受体相关蛋白;双向电泳显示,肾上腺切除哮喘模型针刺组血清中有两个明显区别于其他组的特有蛋白点(MW=17.3kD,PI<4.5和16.9kD,PI<4.5)。质谱分析表明依次为亲环蛋白A、锌指蛋白91。结论:针刺治疗哮喘,是整体调整的多靶点效应,与免疫调节、基因表达、蛋白质合成等密切相关。针刺应答蛋白质功能有待深入研究。

关 键 词：Acupuncture Therapy ASTHMA ELECTROPHORESIS PROTEOME RATS 

分 类 号：R2-03[医药卫生—中医学]