双氢青蒿素干扰前列腺癌PC-3细胞体外生长的研究  

Study on growth interference of dihydroartemisinin in PC-3 cell lines of prostate cancer in vitro

作　　者：高小玲[1] 李庆春[1] 罗子国[1] 

机构地区：[1]重庆医科大学生命科学院,重庆400016

出　　处：《中国医院药学杂志》2009年第24期2074-2077,共4页Chinese Journal of Hospital Pharmacy

摘　　要：目的:研究双氢青蒿素(DHA)对前列腺癌PC-3细胞的增殖、细胞周期和凋亡的影响,探讨DHA干扰前列腺癌PC-3细胞生长的可能机制。方法:应用四甲基偶氮唑盐(MTT)、流式细胞术(FCM)、扫描电镜(SEM)等方法检测不同浓度DHA作用不同时间对前列腺癌PC-3细胞的生长影响。结果:DHA对前列腺癌PG-3细胞的增殖抑制率与DHA浓度和作用时间相关,细胞被阻滞于G_2/M期,诱导细胞凋亡发生。结论:DHA能有效抑制前列腺PC-3细胞增殖并诱导PC-3细胞凋亡。OBJECTIVE To study the effect of dihydroartemisinin（DHA） on the proliferation, cell cycle distribution, apoptosis of PC-3 cells and its possible mechanism. METHODS MTT, flow cytometry（FCM）, scanning electron microscopy （SEM） were employed to observe the effects of DHA of different concentration treating for different times on PC 3 cells. RESULTS Proliferative inhibitorg ratios of PC 3 treated with DHA showed time and concentration dependent manner. G2/M cell cycle arrest and apoptosis was induced. CONCLUSION DHA could effectively inhibit the proliferation of PC-2 and induce its apoptosis.

关 键 词：双氢青蒿素 前列腺癌 细胞周期 凋亡 

分 类 号：R979.1[医药卫生—药品] R737.2[医药卫生—药学]