山楂海棠嫩茎直接再生芽苗体系及种质离体保存  

Preliminary Study on Shoots Regeneration from Tender Stems of Malus komarovii (Sarg.) Rehd. and Germplasm Reservation in vitro

作　　者：顾地周[1] 高捍东[2] 张庆增[1] 王玉方[3] 冯颖[1] 

机构地区：[1]通化师范学院生物系,吉林通化134002 [2]南京林业大学森林资源与环境学院,江苏南京210037 [3]农业部特种经济动植物及产品质量监督检验测试中心,吉林吉林132109

出　　处：《林业科学研究》2009年第6期888-892,共5页Forest Research

基　　金：吉林省科技厅资助项目(200705C05)

摘　　要：The tender stems of Malus komarovii were used as explant and the suitable medium compositions were screened through uniform design experiments.The results showed that tissue culture of M.komarovii required different kinds of culture medium in different phases.SH+TDZ 2.45 mg·L-1+NAA 0.06 mg·L-1 for shoots regeneration immediately at base of tender stem,the rate of induction was 98.8%;1/4 SH+KT 0.35 mg·L-1+NAA 0.05 mg·L-1 for rooting,the rate of rooting was more than 97%;1/8 SH+ABA 2.05 mg·L-1+KT 0.50 mg·L-1 for germplasm preservation in vitro,the rate of dormancy was 95.5%.These plant materials could be maintained for 39 months by the methods of promoting dormancy and low nutrients at normal temperature.The tender stems of Malus komarovii were used as explant and the suitable medium compositions were screened through uniform design experiments. The results showed that tissue culture of M. komarovii required different kinds of culture medium in different phases. SH ＋ TDZ 2.45 mg · L^-1 ＋ NAA 0.06 mg · L^-1 for shoots regeneration immediately at base of tender stem, the rate of induction was 98.8% ; 1/4 SH ＋ KT 0.35 mg · L^-1 ＋ NAA 0.05 mg · L^-1 for rooting, the rate of rooting was more than 97% ; 1/8 SH ＋ABA 2.05 mg · L^-1 ＋KT0.50 mg · L^-1 for germplasm preservation in vitro, the rate of dormancy was 95.5%. These plant materials could be maintained for 39 months by the methods of promoting dormancy and low nutrients at normal temperature.

关 键 词：山楂海棠 植株再生 种质离体保存 均匀设计 

分 类 号：S663.1[农业科学—果树学] S661.5[农业科学—园艺学]