野桑蚕性信息素结合蛋白(PBP)亚家族基因的克隆与进化分析  

Molecular Cloning and Evolution Analysis of Pheromone-binding Protein(PBP) Subfamily Genes From Wild Silkworm Bombyx mandarina

作　　者：张升祥[1] 崔为正[1] 王更先[2,3] 孔令斐[3] 徐世清[3] 

机构地区：[1]山东农业大学林学院,山东泰安271018 [2]邯郸学院生物科学系,河北邯郸056005 [3]苏州大学基础医学与生物科学学院,江苏苏州215123

出　　处：《蚕业科学》2009年第4期776-782,共7页ACTA SERICOLOGICA SINICA

基　　金：国家重点基础研究发展规划"973"项目(编号2005CB1-21005);现代农业产业技术体系建设专项

摘　　要：蛾类的性信息素信号传递是研究昆虫化学通讯的模型,性信息素结合蛋白(pheromone-binding protein,PBP)亚家族是研究热点之一。克隆了野桑蚕PBP亚家族的3个基因,命名为pbp1、pbp2、pbp3(GenBank登录号:GQ246497、GQ468569、GQ468570)。分析野桑蚕和家蚕pbp基因的编码区,发现碱基突变主要以转换为主(14/18),氨基酸变异主要为同义突变(16/18),成熟蛋白质的理化性质变化小,二级结构无变化,推测野桑蚕向家蚕的进化过程中PBP亚家族的基因功能没有变化。对具有PBP亚家族3个已知基因的6个昆虫物种进行氨基酸遗传距离和同源性分析,结果表明野桑蚕和家蚕间PBP1、PBP2、PBP3的遗传距离分别为0、0.02、0,同源性分别高达100%、98.6%和100%,物种间基因的进化速率很慢,进化分析显示PBP亚家族在这几个物种分化前已经产生。Sex pheromone signal transduction in moths is a good model for studying chemical communication between insects.Pheromone-binding protein（PBP） subfamily has received increased attention in this area.In present study,we cloned 3 genes of PBP subfamily,namely pbp1,pbp2 and pbp3（GenBank numbers GQ246497,GQ468569 and GQ468570） from wild silkworm Bombyx mandarina.The results of sequence analysis to coding regions revealed that transversion was the major form of base mutations（14/18） and synonymous mutation occurred predominantly to amino acid residues（16/18）.None or negligible change occurred to physicochemical properties and no change was observed in secondary structures of the mature proteins.It was inferred that there was no functional variance of B.mori PBP subfamily genes in the evolutionary process from B.mandarina.Genetic distance and homology analysis to amino acid sequences of 3 PBP subfamily genes from 6 insect species showed that the genetic distance was 0,0.02 and 0,and the sequence identity was 100%,98.6% and 100% respectively in PBP1,PBP2 and PBP3 between the two closely related species,showing a very low evolutionary rate.Phylogenetic analysis revealed that the PBP subfamilies had already existed before divergence of these 6 insect species.

关 键 词：野桑蚕 家蚕 性信息素结合蛋白 亚家族基因 进化 

分 类 号：S885.9[农业科学—特种经济动物饲养] Q78[农业科学—畜牧兽医]