桑树内生拮抗细菌Lu10-1对家蚕白僵病的防治效果及抑菌作用  

Control Effect and Fungistasis of Mulberry Endogenous Antagoni stic Bacterial Strain Lu10-1 on Silkworm White Muscardine

作　　者：董法宝[1] 高绘菊[1] 王彦文[1] 冀宪领[1] 于奇[1] 牟志美[1] 

机构地区：[1]山东农业大学林学院,山东泰安271018

出　　处：《蚕业科学》2009年第4期803-810,共8页ACTA SERICOLOGICA SINICA

基　　金：山东省自然科学基金项目(编号2007ZRB01872);山东省科技攻关项目(编号2007GG20009014);现代农业产业技术体系建设项目;公益性行业(农业)科研专项(编号nyhyzx07-020-08)

摘　　要：为了开发新型微生物农药用于家蚕病害防治,研究了桑树内生拮抗细菌洋葱伯克霍尔德氏菌(Burkholde-ria cepacia)Lu10-1菌株对家蚕白僵病的防治与抑菌效果。防治试验表明,Lu10-1菌株发酵上清液对家蚕白僵病的预防及治疗有效率分别达到55.88%、34.00%,且对家蚕生理及茧质无不良影响。体外抑菌试验表明,Lu10-1菌株的抗菌粗提物对白僵菌有较强的拮抗作用,抑菌圈直径为17.00 mm;抗菌粗提物还能够抑制白僵菌的生长及孢子萌发,当其作用于白僵菌孢子后,孢子出现聚集、内容物分布不均、变形、空腔及胞壁断裂等异常,作用于菌丝后,菌丝出现变粗、扭曲、皱褶、膨大、内容物流出、消融等现象;抑菌率随着抗菌粗提物浓度的增加而不断增大。初步认为Lu10-1菌株可以用于家蚕白僵病的防治。For developing a new microbial pesticide to control silkworm diseases,the control effect and fungistasis of the antagonistic bacterium Burkholderia cepacia Lu10-1,isolated from mulberry,on silkworm muscardine were investigated.The results of control tests indicated that the supernatant of Lu10-1 zymotic fluid achieved 55.88% control efficiency and 34.00% prophylactic effect on silkworm white muscardine respectively,while no harmful effect was observed on the physiological functions and cocoon quality of the silkworm.The results of inhibitory tests indicated that the antibacterial crude extract of Lu10-1 had strong antagonistic activity against Beauveria bassiana.Its diameter of inhibition zone reached 17.00 mm.The antibacterial crude extract could also inhibit growth and spore germination of Beauveria bassiana.After being treated with the antibacterial crude extract,the spores of Beauveria bassiana became collective and had such abnormalities as uneven distribution of cellular contents,deformation of spores,disappear of cellular contents and disrupted cell wall;and the mycelia of Beauveria bassiana became thicker,retorted,corrugated,and inflated with inner substance outflowed and ablation of the cells.The inhibition efficiency increased with the concentration of antibacterial crude extract.It is preliminarily concluded that the Lu10-1 strain can be used to control silkworm white muscardine.

关 键 词：洋葱伯克霍尔德氏菌Lu10-1 菌株发酵液 抗菌粗提物 家蚕白僵病 防治效果 抑菌作用 

分 类 号：S884[农业科学—特种经济动物饲养]