桑椹与叶片蛋白质双向电泳样品制备方法的比较试验  

Comparative Tests on Preparation Method of Protein Samples From Mulberry Fruit and Leaf for Two-dimensional Electrophoresis

作　　者：王海玲[1] 池旭娟[1] 阚雪芹[1] 方向明[1] 刘艳艳[1] 谈建中[1,2] 

机构地区：[1]苏州大学建筑与城市环境学院,江苏苏州215123 [2]苏州大学蚕桑研究所,江苏苏州215123

出　　处：《蚕业科学》2009年第4期847-850,共4页ACTA SERICOLOGICA SINICA

基　　金：苏州市农业科技计划项目(编号SNG0719);公益性行业(农业)科研专项(编号nyhyzx07-020-03)

摘　　要：为建立适合于桑树蛋白质组研究的双向电泳技术体系,以桑品种"大10"的桑椹与叶片为材料,探讨了桑树蛋白质不同提取方法对凝胶电泳效果的影响。结果表明,改良酚提取法与常规的SDS提取法、尿素/硫脲提取法相比,电泳图谱清晰,分离效果良好,能检测到更多的蛋白质组分,适用于桑椹与叶片蛋白质IPG-IEF/SDS-PAGE双向电泳及SDS-PAGE电泳的样品制备。In order to develop two-dimensional electrophoresis technology suitable for mulberry proteome analysis,the effects of various protein extraction methods on gel electrophoresis were investigated by using fruit and leaves of a mulberry variety named ＂Da 10＂.The results showed that the modified phenol extraction method could yield clearer electrophoretogram and better separation effect over the conventional SDS extraction method and urea/thiourea extraction method.With the modified phenol method,more protein components could be detected,being a suitable method for protein sample preparation from mulberry fruit and leaf for two-dimensional IPG-IEF/SDS-PAGE and SDS-PAGE.

关 键 词：桑椹 桑叶 双向电泳 蛋白质样品 

分 类 号：S663.9[农业科学—果树学]