莪术油诱导K-562细胞凋亡分子机制的实验研究  

Apoptosis of K-562 cells induced by oleum curcumae wenchowensis and its mechanisms

作　　者：刘佳[1] 沈群[1] 季建敏[1] 朱光荣[1] 章亚成[1] 姜鹏君[1] 孙晓超[1] 

机构地区：[1]南京中医药大学第一附属医院血液科,江苏南京210029

出　　处：《中国生化药物杂志》2009年第6期390-392,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：江苏省卫生厅"科教兴卫工程"医学重点人才课题(RC2007068);江苏省"六大人才高峰"第五批高层次人才项目(2008年)

摘　　要：目的探讨莪术油(OCW)诱导慢性粒细胞白血病(CML)细胞株K-562凋亡及可能的分子机制。方法以不同浓度OCW(0,2.5,5,10和20 mg/mL)处理K-562细胞24 h后,光镜下观察形态学改变;CCK-8法检测细胞增殖抑制率;Hoechest33258荧光染色及FITC-AnnexinⅤ/PI双染法检测细胞凋亡率;半定量RT-PCR及Western blot方法检测bcr/abl、bcl-2、p53、Fas/FasL表达的变化。结果OCW明显抑制K-562细胞增殖,诱导细胞凋亡,与浓度呈正相关;各组药物干预后Fas/FasL基因在mRNA和蛋白水平呈浓度依赖性上调,而bcr/abl、bcl-2、p53基因表达无明显变化。结论不同浓度OCW能有效诱导K-562细胞凋亡,其作用可能通过Fas/FasL途径而实现的,与bcr/abl、bcl-2、p53基因无明显相关。Purpose To investigate the effects and the mechanism of oleum curcumae wenchowensis （OCW） with different concentrations （0, 2.5, 5, 10 and 20 mg/mL） on chronic myeloid leukemia cell line K-562 cells in vitro. Methods The apoptosis of K-562 cells was dyed by Hoechest 33258 and detected by flow cytometry marked with Annexin V/PI. The Expression of Fas/FasL, bcr/abl, bcl-2 and p53 was detected by semi-quantity reverse transcript-polymerase chain reaction （RT-PCR） and Western blot. Results The results showed that the apoptosis rates were gradually elevated. The expression of Fas and FasL protein was increased in a concentration dependent manner, while bcr/abl, bcl-2 and p53 had no significant changes. Conclusion OCW could induce the apoptosis of K-562 ceils by up-regulating the expression of Fas/FasL protein.

关 键 词：莪术油 K-562细胞 细胞凋亡 FAS/FASL 

分 类 号：R979.1[医药卫生—药品] R963[医药卫生—药学]