HLA-DRB1、DQB1基因多态性与流行性出血热的相关性  

Association of HLA-DRB1,-DQB1 alleles with epidemic hemorrhagic fever

作　　者：冯继红[1] 孙万邦[1] 罗军敏[1] 李维宏[2] 黄学贵[1] 张忆雄[1] 

机构地区：[1]遵义医学院珠海校区,广东珠海519041 [2]重庆医科大学附属第一医院

出　　处：《山东医药》2009年第48期23-25,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30360099)

摘　　要：目的从基因水平探讨HLA-DRB1、DQB1等位基因多态性与流行性出血热的相关性,以阐述其免疫遗传学特征。方法应用序列特异性引物聚合酶链反应技术检测流行性出血热患者和正常对照组各50例的HLA-DRB1、DQB1等位基因。结果流行性出血热患者与正常对照组比较,HLA-DRB1*16等位基因分布频率明显增高(Pc=0.010 6),两组间其余HLA-DRB1-、DQB1等位基因分布频率差异无统计学意义(Pc>0.05)。结论HLA-DRB1*16等位基因与流行性出血热呈正相关,可能为流行性出血热易感基因之一。Objective To explore the hereditary susceptibility of HLA-DRB1 and -DQB1 alleles of epidemic hemorrhagic fever （EHF） at gene level and to further the features of their immunogenetics. Methods The polymorphisms of HLA-DRBI and -DQB1 alleles were typed by sequence specific primer based on polymerase chain reaction in 50 patients with EHF and 50 normal control subjects. Results The allele frequency of HLA-DRBI ＊ 16 allele was significantly higher in patients than that in normal controls （Pc =0.010 6）. There was no significantly difference between the rested alleles of HLA-DRB1 and -DQBI in two groups （Pc 〉0.05）. Conclusions HLA-DRB1 ＊ 16 allele was initially be determined predisposing gene of EHF.

关 键 词：流行性出血热 序列特异性引物聚合酶链反应 人类白细胞抗原 相关性 

分 类 号：R512.81[医药卫生—内科学]