单双侧电针治疗CCI大鼠对脊髓背角P物质影响的比较  

Comparison of The Effects of Single and Bilateral Sides Electro-acupuncture on SP in Spinal cord of CCI Model Rat

作　　者：王金金[1] 屠文展[1] 蒋松鹤[1] 余清[2] 谢丽微[1] 楼新法[2] 

机构地区：[1]温州医学院附属第二医院,浙江温州325027 [2]温州医学院,浙江温州325035

出　　处：《中华中医药学刊》2009年第12期2576-2579,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省教育厅资助项目(20051157);温州市科技局科研基金资助项目(Y20080031)

摘　　要：目的:观察单双侧电针对坐骨神经慢性挤压伤大鼠脊髓背角P物质(SP)表达的影响。方法:35只雄性SD大鼠造模后随机分为假模组(J组)、对照组(A组)、患侧电针组(B组)、健侧电针组(C组)、双侧电针组(D组)。各电针组治疗9天后,观察电针前后痛阈变化;免疫组化法检测脊髓背角SP的表达。结果:B、C、D组之间比较,D组大鼠脊髓背角中SP阳性反应物总IOD值较B、C两组均有不同程度减少(P<0.01)。B、C组之间大鼠脊髓背角中SP阳性反应物总IOD值B组<C组(P<0.01)。结论:电针可能通过抑制大鼠脊髓背角中SP的含量,产生镇痛作用,其中以双侧电针镇痛作用最好。Objective ： To observe the expression changes of Substance P in spinal cord of chronic constriction injury of the sciatic nerve model rats by single side and bilateral side electro - acupuncture. Methods ： After CCI model was successfully made, seventy male Sprague - Dawley rats were randomized into false group （ Group J）, control group （ Group A）, sick side dectro - acupuncture group （ Group B）, healthy side electro - acupuncture group（ Group C） ,bilateral side electro - acupuncture group（ Group D） . After every electro - acupuncture group was treated 9 days, observe the changes of pain threshold before and after the electro - acupuncture ; And detected the expressing of SP in Spinal dorsal horn by using immunohistochemistry. Results ：Compared within group B. C. D,The total IOD value of SP positive material in Spinal dorsal horn of rats in Group D decreased to various degrees compared with Group B and C （P 〈0.01 ） ;The total IOD value of SP positive material in Spinal dorsal horn in rats of Group B was less than Group C （P 〈 0.01 ）. Conclusion：Electro - acupuncture therapy may be through suppressing substance P content in rats spinal dorsal horn ; and generated the analgesia function,within all groups, bilateral side electroacupuncture has the most obvious analgesic effect.

关 键 词：电针 坐骨神经痛 P物质 痛阈 

分 类 号：R245.97[医药卫生—针灸推拿学]