甘草酸二铵磷脂复合物对急慢性肝损伤保护作用的研究  

Protective Effects of Diammonium Glycyrrhizinate Phospholipid Complex on Acute and Chronic Liver Injury

作　　者：陈晓瑾[1] 茹仁萍[1] 尤琳雅[1] 李松龙[1] 王琪[1] 宋洁[1] 

机构地区：[1]杭州市第六人民医院药剂科,杭州浙江310014

出　　处：《中华中医药学刊》2009年第12期2636-2639,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：杭州市科技局科研项目(20051333838)

摘　　要：目的:评价甘草酸二铵磷脂复合物注射剂(DG-PC)的对急性、慢性免疫性肝脏损害的保护作用。方法:大鼠腹腔注射四氯化碳、C57BL/6 J小鼠注射氨基半乳糖和Freund完全佐剂(FCA)分别复制急性和慢性肝脏损害模型,测定血清ALT、AST,并对肝脏进行病理学观察。结果:DG-PC能降低急性肝损大鼠ALT水平(P<0.05)和慢性肝损伤小鼠的AST水平(P<0.01)。急性肝损伤大鼠肝组织评分结果显示DG-PC能显著降低四氯化碳染毒肝脏组织的病理评分(P<0.01),DG和模型组没有差异。肝组织切片光镜和电镜观测均显示,DG-PC可以改善急慢性肝损伤导致的肝细胞变性和损伤。结论:DG-PC对于大鼠急性化学性肝损伤和C57BL/6 J小鼠慢性免疫性肝损伤均具有一定的保护作用。Objective：To investigate the protective effects of diammonium glycyrrhizinate phospholipid complex injection （ DG - PC） on acute and chronic - immunological liver injury. Methods ： Acute hepatic injury rat model was established by intrapeHtoneal （ip） injection with carbon tetrachloride （CC14 ） ,chronic -immunological hepatic liver was produced by D - galactosamine combined with Frcunds＇complete adjuvant （FCA）. ALT and AST were assayed in both models,as well as hepatic tissue pathology examination. Results： The liver function results showed that DG - PC can lower ALT after acute liver injury （P 〈 0.05 ） , and significantly decrease AST after chronic liver injury （P 〈 0.01 ）, whiles DG has no difference. DG - PC can significantly lower score than DG compared to the model group on pathology examination （P 〈 0. 01 ）. Hepatic pathology study showed DG- PC could alleviate hepatic inflammation and effectively protect liver cells. Conclution： Diammonium glycyrrhizinate phospholipid complex injection has protective effects on acute hepatic injury induced by CC14 in rat, as well as chronic and immunological liver damage induced by D - Gal/FCA in mice.

关 键 词：甘草酸二铵磷脂复合物 急性肝损伤 慢性免疫性肝损伤 病理 超微结构 

分 类 号：R285.5[医药卫生—中药学]