RP-HPLC法测定罗布麻叶中芦丁和槲皮素的含量  

Determination of Rutin and Quercetin in Folium Apocyni Venety by RP-HPLC

作　　者：刘唯芬[1] 慕善学[1] 刘欣荣[1] 冯川[1] 

机构地区：[1]沈阳药科大学高等职业技术学院,辽宁沈阳110026

出　　处：《中华中医药学刊》2009年第12期2677-2678,共2页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的：建立高效液相色谱法测定罗布麻叶中芦丁和槲皮素的含量。方法：采用krom asil C18柱（4.6mm×250 mm,5μm）;流动相为甲醇（A）-0.08%磷酸（B）,梯度洗脱;流速1.0 mL/m in;检测波长360nm。结果：芦丁进样量在0.42~7.10μg范围内（r=0.9998）,槲皮素进样量在0.011~0.19μg范围内（r=0.9997）线性关系良好,平均回收率芦丁为98.77%（RSD%=1.2）,槲皮素为99.19%（RSD%=1.8）。结论：该方法操作简便,准确,重复性好,可同时测定罗布麻叶中芦丁和槲皮素的含量。Objective：To develop an HPLC method for determination of rutin and quercetin in Foliuna apocyni venety. Methods ： Analyses were carried out using a kromasil Cls column （ 4.6 mm×250 mm,5μm ）. The mobile phase was A （methanol） - B（0.08% phosphoric acid solution） with gradient elution and the flow rate was 1.0mL/min ,the UV detector was set at 360nm. Results ： A linear relationship of rutin was good at the range of 0.42 - 7. 10μg （ r = 0. 9998） and quercetin was good at the range of 0.011 ~ 0. 19μg （ r = 0.9997 ）. The average recovery of rutin was 98.77% （ RSD% = 1.2） and of quercetin was 99.19% （RSD% = 1.8 ）. Conclusion：The method is simple, accurate and good reproducibility. It is suitable for the simultaneous determination of rutin and quercetin in Folium apocyni venety.

关 键 词：高效液相色谱法 罗布麻叶 芦丁 槲皮素 

分 类 号：R285.5[医药卫生—中药学]