^(125)I标记氧化低密度脂蛋白抗体  

Labeling of OxLDL-Ab With ^(125)I

作　　者：刘子华[1] 邓新荣[1] 徐燕华 罗志福[1] 

机构地区：[1]中国原子能科学研究院同位素研究所,北京102413 [2]成都华神生物技术有限责任公司,四川成都610225

出　　处：《同位素》2009年第4期204-208,共5页Journal of Isotopes

摘　　要：采用Iodogen法对氧化低密度脂蛋白抗体(OxLDL-Ab)进行了125I标记,并对标记条件进行了优化;采用PD-10柱对标记抗体进行分离纯化,并对125I-OxLDL-Ab的体外稳定性和活性进行评价。结果表明,125I-OxLDL-Ab标记率>80%,放化纯度>95%,比活度达73.3 TBq/g;125I-OxLDL-Ab分别在生理盐水、含2%BSA的生理盐水和人血清中4℃放置96 h,放化纯度降低均小于5%;125I-OxLDL-Ab在体外与OxLDL结合的Ka为10.6±1.3 GL/mol。以上结果提示125I-OxLDL-Ab体外稳定性好,与OxLDL结合活性高,125I标记对其活性影响小,可以用于体外放免诊断试剂盒的研究。OxLDL-Ab was labeled with ^ 125I by the Iodogen method, and the labeling was purified by PD-10 column. The optimal condition of radiolabeling reaction was determined. The stability and affinity to OxLDL of ^125 I-OxLDL-Ab in vitro were evaluated as well. The labeling yield of ^125 I-OxLDL-Ab was more than 80 % and the radiochemical purity was more than 95 %. The results of in vitro stability studies showed that the radiochemical purity of ^125I-OxLDL-Ab declined less than 5% after 96 h storage under following condition： 0.9M NaCl, 2%BSA in 0.9%NaCl solution and human serum at 4℃ respectively. In the binding experiments of ^125I-OxLDL-Ab and OxLDL, Ka was （1.06±0.13）× 10^10 L/mol. The results showed that ^125I-OxLDL-Ab had high stability and high affinity to OxLDL, so it was worthy of further research for in vitro diagnosis kit.

关 键 词：125I 氧化低密度脂蛋白单克隆抗体(OxLDL-Ab) 标记 

分 类 号：R817[医药卫生—影像医学与核医学]