光照及黑暗条件下不同浓度2,4-D对诱导苦马豆愈伤组织的影响  

Effects of Different Concentration of 2,4-D on Callus Induction of Swainsona Salsula under Bright or Dark Conditions

作　　者：周吉林[1] 伍春莲[2] 陈卫英[2] 

机构地区：[1]广安职业技术学院生物系,四川广安638000 [2]西华师范大学生命科学学院,四川南充637002

出　　处：《内江师范学院学报》2009年第12期41-44,共4页Journal of Neijiang Normal University

基　　金：四川省教育厅青年基金项目(07ZB089)

摘　　要：以苦马豆幼苗的子叶及下胚轴为外植体,研究了光照及黑暗条件下不同浓度2,4-D对苦马豆愈伤组织生长量的影响.结果表明:子叶愈伤组织的生长均优于下胚轴愈伤组织的生长,2,4-D浓度为0.3,0.6mg/L时,子叶、下胚轴愈伤组织的生长光培养优于暗培养;在2,4-D浓度为1.2mg/L时,子叶、下胚轴愈伤组织的生长暗培养优于光培养.因此,以2,4-D单激素诱导苦马豆愈伤组织,子叶培养在暗处是最好的选择.Swainsona salsula is an important medicinal plant. Using the cotyledons and hypoeotyls of Swainsonia salsula as explants, the effect of different concentration of 2,4-D on callus induction of Swainsona salsula＇s growth under bright or dark conditions was researched,. The results showed that the callus＇s growth of the cotyledons was greater than the hypoeotyls＇s. In 0.3mg/L, 0.6mg/L 2, 4-D, bright conditions were found to have a better result than dark conditions ~ in 1.2mg/L 2, 4-D, better results were observed under dark conditions than under bright conditions. Therefore, if the callus of Swainsona salsula was induced by 2, 4-D, the growth of cotyledons was better under dark conditions.

关 键 词：2 4-D 苦马豆 愈伤组织 鲜重 生物量 

分 类 号：Q813.12[生物学—生物工程]