花脸蘑(Lepista sordida)蛋白粗提液抗烟草花叶病毒活性研究  

Study on the activity of anti-tobacco mosaic virus of crude protein from Lepista sordida

作　　者：马凤金[1,2] 燕继晔[2] 严红[2] 张丽叶[1] 李兴红[2] 

机构地区：[1]北京化工大学,北京100029 [2]北京市农林科学院植物保护环境保护研究所,北京100097

出　　处：《河北农业大学学报》2009年第6期92-96,共5页Journal of Hebei Agricultural University

基　　金：北京市科委重大项目(D070605040431);国家科技支撑计划(2007BAD87B01-2)

摘　　要：以花脸蘑蛋白粗提液为对象,研究了其诱导烟草对TMV的抗性,对TMV的体外钝化作用及对TMV引起的番茄病毒病的室内控制作用;探讨了浓度、温度、pH值对花脸蘑蛋白粗提液抗TMV活性的影响。结果表明：当粗提液浓度为100μg/mL时,诱导烟草抗TMV的侵染枯斑抑制率可达83.97%,对TMV的体外钝化作用的枯斑抑制率为74.57%,对番茄病毒病的室内控制效果达到82.70%,表明花脸蘑蛋白粗提液通过诱导抗病性和对TMV的体外钝化作用达到了控制TMV的作用;花脸蘑蛋白粗提液具有很好的温度稳定性,在50℃的条件下处理30 min,抗TMV侵染的枯斑抑制率仍达66.30%;花脸蘑蛋白粗提液也具有很好的pH值稳定性,pH敏感性试验表明,在pH为4.010.0的条件下,都具有抗TMV活性,在中性（pH=8）的条件下,活性最强可达81.50%。The crude protein extracted from Lepista sordida was studied,which can induce tobacco resistance to TMV,anti-TMV in vitro and control tomato virus disease caused by TMV.The effect on the anti-TMV activity of the protein by its concentration,temperature and pH were also studied.The results showed that when the concentration is 100μg/mL,the inhibition ratio of induced resistance to TMV and anti-TMV in vitro were 83.97% and 74.57%,respectively,the control ration of tomato virus disease was 82.70% in the greenhouse.When the crude protein was bathed at 50 ℃ for 30 min,the inhibition ratio of anti-TMV was 66.30%,and the results of pH test showed that crude protein from Lepista sordida can keep a high activity of anti-TMV between pH4.0-10.0,and its inhibition ratio of anti-TMV is 81.50% at pH8.0,which is the optimum pH.

关 键 词：花脸蘑 蛋白粗提液 TMV 番茄病毒病 

分 类 号：S432.26[农业科学—植物病理学]