氧化应激对离体大鼠小肠上皮细胞的损伤及抗氧化研究  

Study of oxidative stress on injury in intestinal epithelial cell and antioxidation in vitro

作　　者：陈群[1] 于维[1] 于秀芳[1] 刘海燕[1] 

机构地区：[1]吉林省农业科学院畜牧分院动物营养研究所,吉林公主岭136100

出　　处：《河北农业大学学报》2009年第6期101-104,共4页Journal of Hebei Agricultural University

摘　　要：为了探讨氧化应激对动物小肠上皮细胞内抗氧化酶活性、细胞通透性和细胞增值的影响,并进一步研究GSH对离体小肠上皮细胞诱导型氧化损伤的保护作用。通过建立大鼠小肠上皮细胞X/XO体外氧化损伤模型,培养细胞随机分为7组,18个重复:对照组A加入蒸馏水,氧化损伤B、C、D各组加入黄嘌呤X(10μmol/L),并分别加入黄嘌呤氧化酶XO,(10,40,70 U/L),抗氧化E、F、G各组加入X(10μmol/L)和谷胱甘肽GSH(1.5μmol/ml),并分别加入XO(10,40,70U/L),应激培养24 h。研究发现:不同剂量的X/XO均可导致离体大鼠小肠上皮细胞氧化应激的发生。与对照组比较,显著降低了IEC增殖和CAT的活性,明显提高细胞内Ca2+浓度(P<0.05)。低活性XO(10U/L)提高了SOD活性(P>0.05),然而高活性XO(40,70 U/L)显著降低了细胞内SOD的活性(P<0.05),添加谷胱甘肽提高了抗氧化酶的活性和细胞增殖,显著降低了细胞内的Ca2+浓度。The objective of this study was to investigate the direct oxidative injury of free radicals on rat intestinal epithelial cells（IEC）,and antioxidation by glutathione（GSH）in vitro.The results show that X/XO supplementation significantly inhibited the proliferation of IEC and activity of catalase（CAT）,but increased the level of Ca^2＋ compared with the control group（P〈0.05）.There was a slight increase in the activity of super oxide dismutase（SOD） by the low concentration of XO（10 U/L） alone-treated group（P〉0.05）,while supplementation of a high concentration of XO（40,70 U/L） significantly decreased the activity of SOD compared with XO（10 U/L） and the control group（P〈0.05）.Presence of GSH failed to affect the activity of SOD corresponding X/XO（10 U/L） alone-treated.The increase in cell proliferation was also observed in IEC.These results strongly suggest that oxidative stress could induce injury in IEC.Moderate oxidative stress might have selectively stimulated the synthesis of antioxidant enzyme,but significantly decreased cell proliferation.Additions of GSH showed significant protection against oxidative stress in vitro.

关 键 词：自由基 抗氧化酶 细胞增殖 

分 类 号：S828.5[农业科学—畜牧学]