刺五加皂甙对PC12细胞缺氧诱导因子-1α表达的影响  

Effect of of ASS on the expression of HIF-1α in PC12 cells

作　　者：周纯[1] 顾永健[1] 姜正林[1] 刘春风[2] 

机构地区：[1]南通大学附属医院,江苏南通226000 [2]苏州大学附属第二医院

出　　处：《山东医药》2009年第47期16-17,共2页Shandong Medical Journal

基　　金：江苏省科技厅社会发展科技计划课题项目(BS2000040)

摘　　要：目的观察刺五加皂甙(ASS)对PC12细胞缺氧诱导因子-1α(HIF-1α)表达的影响,探讨其抗缺血缺氧脑损伤作用的机制。方法将PC12细胞分为三组,对照组在无血清的DMEM培养基中培养;模型组加入终浓度为125μmol/L的CoCl2共同作用4 h;ASS组先用终质量浓度为50μg/ml的ASS预处理24 h,再加入终浓度为125μmol/L的CoCl2共同作用4 h。采用MTT比色分析法测定各组PC12细胞活性,Western Blot法检测HIF-1α表达的变化。结果对照组、模型组、ASS组细胞活性OD值分别为0.538±0.027、0.320±0.045、0.615±0.039;HIF-1α表达灰度比值分别为0.085±0.033、0.782±0.050、0.976±0.070。各组间相比,P均<0.01。结论ASS对缺血缺氧性脑损伤有保护作用,其机制与诱导HIF-1α表达有关。Objective To observe the effects of acanthoparmx senticosus saponins （ASS） on the expression of hypoxic inducible factor-1 （HIF-1α） in PC12 cells and discuss its mechanism of anti-ischemia-hypoxia injury. Methods PC12 cells were divided into three groups ,the control group was cultured in serum-free DMEM media, the model group was performed with 125μmol/L CoCl2 for 4 h, the ASS group was performed with 50 g/ml ASS for 24 h and 125μmol/L CoCl2 for 4h. The viability of PC12 cells was determined by MTT assay,the expression of HIF-1α induced was detected by Western blot. Results The viability（OD value ） of PC12 cells in the control group,model group and ASS group were 0. 538±0. 027,0.320±0.045,0.615± 0.039 respectively, the expression of HIF-1α （ ratio of gray value ） were 0. 085 ± 0. 033,0. 782 ± 0. 050,0. 976 ±0.070 respectively. There were significant differences among the three groups （P 〈 0.05 ） . Conclusions ASS can protect brain ceils from ichemia injury, the possible mechanism maybe related to the up-regulation of HIF- 1α activation.

关 键 词：刺五加 刺五加皂甙 脑血管病 缺氧诱导因子-1Α 

分 类 号：R941[医药卫生—药剂学]