Effects of salinity fluctuation amplitudes on growth,osmolarity,Na^+-K^+-ATPase activity and Hsp70 of juvenile Chinese shrimp Fenneropenaeus chinensis Osbeck  

作　　者：丁森 王芳 董双林 高勤峰 

机构地区：[1]The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China

出　　处：《Chinese Journal of Oceanology and Limnology》2009年第4期723-728,共6页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No. 30571441);Reward Research Foundation for Talented Young and Middle Aged Scientists of Shandong,China (No. 2006BS07002);National Key Technology Research and Development Program of China (No. 2006BAD09A07)

摘　　要：The effects of various salinity fluctuation amplitudes （2, 4, 6 and 8） on the growth, osmolarity, Na＋-K＋-ATPase activity and Hsp70 of juvenile Fenneropenaeus chinensis cultured in seawater with a salinity of 20 were studied. The results show that weight gain in the salinity fluctuation treatments was better than that in control; in particular, the weight gain of treatments S4 and SO, at 231.8% and 196.3%, respectively, was significantly different （P〈0.05）. The hemolymph osmolarity of treatments SO, S2, S4, S6 and S8 was 635.4, 630.8, 623.6, 614.4 and 600.3 mOsm/kg, respectively, and decreased with increasing salinity fluctuation amplitude. The level of Na＋-K＋-ATPase activity in gills ofE chinensis was higher than that in hepatopancreas, but there were no significant differences among all treatments, either in gills or hepatopancreas （P〉0.05）. The relative level of Hsp70 in treatment $4 was 48.4% and 40.4% higher than control in muscle and eyestalks, respectively, with the highest values being recorded under a salinity fluctuation amplitude of 4.The effects of various salinity fluctuation amplitudes (2,4,6 and 8) on the growth,osmolarity,Na+-K+-ATPase activity and Hsp70 of juvenile Fenneropenaeus chinensis cultured in seawater with a salinity of 20 were studied.The results show that weight gain in the salinity fluctuation treatments was better than that in control;in particular,the weight gain of treatments S4 and S0,at 231.8% and 196.3%,respectively,was significantly different (P<0.05).The hemolymph osmolarity of treatments S0,S2,S4,S6 and S8 was 635.4,630.8,623.6,614.4 and 600.3 mOsm/kg,respectively,and decreased with increasing salinity fluctuation amplitude.The level of Na+-K+-ATPase activity in gills of F.chinensis was higher than that in hepatopancreas,but there were no significant differences among all treatments,either in gills or hepatopancreas (P>0.05).The relative level of Hsp70 in treatment S4 was 48.4% and 40.4% higher than control in muscle and eyestalks,respectively,with the highest values being recorded under a salinity fluctuation amplitude of 4.

关 键 词：Fenneropenaeus chinensis GROWTH OSMOLARITY Na＋-K＋-ATPase activity HSP70 salinityfluctuation amplitude 

分 类 号：S917.4[农业科学—水产科学]