Effects of tributyltin (TBT) on enzyme activity and oxidative stress in hepatopancreas and hemolymph of small abalone,Haliotis diversicolor supertexta  

作　　者：贾锡伟 张子平 王淑红 林鹏 邹志华 黄邦钦 王艺磊 

机构地区：[1]State Key Laboratory of Marine Environmental Science,Ministry of Education,Environmental Science Research Center,Xiamen University [2]The Key Laboratory of Science and Technology for Aquaculture and Food Safety,Fisheries College,Jimei University

出　　处：《Chinese Journal of Oceanology and Limnology》2009年第4期816-824,共9页中国海洋湖沼学报（英文版）

基　　金：Supported by the Committee of Xiamen Science and Technology,Xiamen,China (No.502Z20055024);the National Natural Science Foundation of China (No.20877034);the Innovation Team Foundation of Jimei University (No.2008A001)

摘　　要：We investigated the effect of tributyltin (TBT) exposure on the concentration of malondialdehyde (MDA) and the activity levels of the superoxide dismutase (SOD),catalase (CAT),and acid and alkaline phosphatase (ACP and AKP) enzymes in the small abalone,Haliotis diversicolor supertexta.We collected samples of the hepatopancreas and hemolymph 2,6,24,48,96,and 192 h after exposure to 0.35 μg (Sn)/L TBT.In the hepatopancreas,ACP activity was significantly higher in animals exposed to TBT 2,24,and 96 h post-exposure compared with the control animals.AKP activity was also higher after 2 h,but SOD and CAT activity was unchanged.The concentration of MDA in the hemolymph was significantly higher than the control animals 2 and 6 h post-exposure.In the hemolymph of animals exposed to TBT,ACP activity was significantly lower than in the control animals 192 h post-exposure,whereas AKP activity was significantly lower 2 and 192 h post-exposure.Hemolymph SOD activity and levels of MDA were significantly lower than in the control animals 24 h after exposure but significantly higher after 96 h.Our results demonstrate that exposure to TBT cause rapid changes in ACP and AKP activity as well as altering the concentration of MDA in the hepatopancreas and hemolymph.SOD and CAT do not appear to be involved in the detoxification of TBT in the hepatopancreas of small abalone.We investigated the effect of tributyltin （TBT） exposure on the concentration of malondialdehyde （MDA） and the activity levels of the superoxide dismutase （SOD）, catalase （CAT）, and acid and alkaline phosphatase （ACP and AKP） enzymes in the small abalone, Haliotis diversicolor supertexta. We collected samples of the hepatopancreas and hemolymph 2, 6, 24, 48, 96, and 192 h after exposure to 0.35 μg （Sn）/L TBT. In the hepatopancreas, ACP activity was significantly higher in animals exposed to TBT 2, 24, and 96 h post-exposure compared with the control animals. AKP activity was also higher after 2 h, but SOD and CAT activity was unchanged. The concentration of MDA in the hemolymph was significantly higher than the control animals 2 and 6 h post-exposure. In the hemolymph of animals exposed to TBT, ACP activity was significantly lower than in the control animals 192 h post-exposure, whereas AKP activity was significantly lower 2 and 192 h post-exposure. Hemolymph SOD activity and levels of MDA were significantly lower than in the control animals 24 h after exposure but significantly higher after 96 h. Our results demonstrate that exposure to TBT cause rapid changes in ACP and AKP activity as well as altering the concentration of MDA in the hepatopancreas and hemolymph. SOD and CAT do not appear to be involved in the detoxification of TBT in the hepatopancreas of small abalone.

关 键 词：tributyltin （TBT） superoxide dismutase （SOD） catalase （CAT） acid and alkaline phosphatase（ACP and AKP） malondialdehyde （MDA） Haliotis diversicolor supertexta 

分 类 号：S917.4[农业科学—水产科学]