Isolation and characterization of venom from nematocysts of jellyfish Rhopilema esculentum Kishinouye  

作　　者：冯金华 于华华 李翠萍 邢荣娥 刘松 王琳 蔡圣宝 李鹏程 

机构地区：[1]Institute of Oceanology,Chinese Academy of Sciences [2]Graduate School,Chinese Academy of Sciences

出　　处：《Chinese Journal of Oceanology and Limnology》2009年第4期869-874,共6页中国海洋湖沼学报（英文版）

基　　金：Supported by Qingdao Science and Technology Project (No. 08-1-3-51- JCH);the Award Foundation of Scientific Research for Excellent Young and Middle-Age Scientist of Shandong Province (No.2006BS 07003)

摘　　要：The present work is the first report of the biochemical characterization of the venom from nematocysts of the jellyfish Rhopilema esculentum Kishinouye. The nematocysts were isolated by autolysis and centrifugation and separated by flow cytometry. Four types of nematocysts were identified： mastigophores, euryteles, and atrichous and holotrichous isorhiza. SDS-PAGE and amino acid analyses demonstrated that most of the proteins in the nematocyst extract were between 10 kDa and 40 kDa, and that glutamic acid was the main amino acid. A hemolytic activity assay showed that the activity of the nematocyst venom （RNV） was strongest in Tris-HCl buffer （50 mmol/L, pH 7.8, 5% glycerol, 0.5 mmol/L EDTA, 0.1 mol/L NaC1）. The hemolytic activity was related to protein concentration and the HU50 against chicken erythrocytes was 0.91 μg/mL.The present work is the first report of the biochemical characterization of the venom from nematocysts of the jellyfish Rhopilema esculentum Kishinouye.The nematocysts were isolated by autolysis and centrifugation and separated by flow cytometry.Four types of nematocysts were identified:mastigophores,euryteles,and atrichous and holotrichous isorhiza.SDS-PAGE and amino acid analyses demonstrated that most of the proteins in the nematocyst extract were between 10 kDa and 40 kDa,and that glutamic acid was the main amino acid.A hemolytic activity assay showed that the activity of the nematocyst venom (RNV) was strongest in Tris-HCl buffer (50 mmol/L,pH 7.8,5% glycerol,0.5 mmol/L EDTA,0.1 mol/L NaCl).The hemolytic activity was related to protein concentration and the HU50 against chicken erythrocytes was 0.91 μg/mL.

关 键 词：NEMATOCYST jellyfish venom Rhopilema esculentum hemolytic activity 

分 类 号：S917.4[农业科学—水产科学]