一株蜀柏毒蛾无芽胞杆菌病原的鉴定与毒力研究  

Identification of Pathogenic Strain of No-Sporeforming to Parocneria orienta and Its Toxicity

作　　者：冯波[1] 周建华[2] 郭亨孝[3] 刘应高[1] 肖育贵[2] 

机构地区：[1]四川农业大学,雅安625014 [2]四川省林业科学研究院,成都610081 [3]四川省林业厅,成都610066

出　　处：《林业科学》2009年第11期104-108,共5页Scientia Silvae Sinicae

基　　金：国家"十一五"科技支撑项目(2006BAD08A1202)

摘　　要：从蜀柏毒蛾暴发种群中发现大量虫体萎缩、体色变红的感病现象。经采集、分离得到一株病原菌,其纯培养物的致病性被科赫原理证明。经生理生化测定和分子鉴定,确定此病原菌为缺陷假单胞菌。通过室内感染测定该菌对蜀柏毒蛾2龄幼虫的毒力,毒力回归方程为Y=-9.7851+6.667X,致死中含量LC50=9.28×108cfu.mL-1。室内和林间感染试验表明:该病原对感染幼虫具有明显的抑食作用,未死亡残存种群在体长、体质量、羽化率、怀卵量和卵的孵化率方面都显著低于对照。A pathogen was isolated from the larva of Parocneria orienta whose body was atrophic and rubescent.Its pathogenicity was testified by law of KOCH.It was identified as Brevundimonas bullata by physiological、 biochemical test and molecular systematic analysis.Toxicity of the bacterium to larva of Parocneria orientia was assayed by means of oral infection.Results showed that the linear regression relationship between the logarithm（y）of the bacteria concentration and the probability（x）of corrected mortality is Y=-9.785 1＋6.667X,and the median lethal concentration is LC_ 50=9.28×108 cfu·mL-1.The result also showed that this pathogen had obvious function of restraining infection larva from taking food.The survival larva were obviously under the antitheses in length of body,avoirdupois,the ratio of eclosion,amount of nit and the ratio of hatch.

关 键 词：无芽孢杆菌 蜀柏毒蛾 毒力研究 

分 类 号：S763.42[农业科学—森林保护学]