弓形体抗原的纯化与分析  

Purification and Analysis of Antigens of Toxoplasma

作　　者：傅翠娥[1] 杨旭明[1] 林爱芬[1] 余毅[1] 张乐[1] 楼涤[1] 陈彩华[1] 

机构地区：[1]浙江省医学科学院寄生虫病研究所

出　　处：《中国畜禽传染病》1998年第5期294-296,共3页

基　　金：淅江省自然基金

摘　　要：对几种提纯弓形体抗原的方法进行了比较。结果，分别经５０００ｒ、１００００ｒ、１５０００ｒ／ｍｉｎ离心３０分钟的粗抗原，用于检测弓形体抗体的Ｐ／Ｎ值依次为５．０５、５．６０、６．４３。用１００００ｒ／ｍｉｎ３０分钟处理的粗抗原（Ｒ１００００），经凝胶柱层析分离洗脱，呈现２个蛋白峰（Ｆ１、Ｆ２），经免疫学测定，其主要的抗原活性部分存在于Ｆ１中。Ｒ１００００抗原用５０％硫酸铵沉淀（Ｓ５０）的蛋白质得率达６４％；用７０％硫酸铵沉淀（Ｓ７０）的蛋白质率为３５％。Ｒ１００００与Ｆ１、Ｆ２、Ｓ５０、Ｓ７０５个抗原，经ＥＬＩＢ后用高免兔血清识别的条带数：Ｆ１和Ｆ２各１条、Ｓ５０Ｚ条、Ｓ７０和Ｒ１００００各３条。除Ｆ２之外，３９～４０ＫＤ为４种抗原的共同反应区带。用上述５种抗原致敏反应检测弓形体抗体，以Ｓ５０具有更高的敏感性和特异性。Several methods for purification of antigens of toxoplasma were compared in this paper The result showed that when the antigens were cetrifuged at 5000 10000 15000r/min for thirty mimutes,the P/N Vale of the detected toxoplasma antibody was 5 05 5 60 6 43 respectively Two protein paks (F 1,F 2)were obtained when the antigen was centrifuged at 10000 r/min (R10000)for thirty minutes by chromatography Immunolog test demonstrated that the major antigen was in F1 The protein recovery of R10000 precipitated by 50% ammonium sulfate (S 50 )was 64% while by 70% ammonium sulfate (S 70 )was 35% By ELIB,umbers of reactive bands were F 1 and F 2 1 band,S 50 2 bands,S 70 and coarse antigen 3 bands Apart from F 2,39～40 KD was the common reactive region of four antigens Above five antigens were coated on plates to detect toxoplasma antibody,the results showed that S 50 had ahigher sensitivity and specifcity than the others.

关 键 词：盐析沉淀 凝胶过滤 弓形体抗原 提纯 免疫学 

分 类 号：S852.43[农业科学—基础兽医学] S855.9[农业科学—兽医学]