超声法提取菝葜中总黄酮的正交实验研究  

Orthogonal Test of Total Flavonoids Extraction from Smilax china L. Jones by Ultrasonic Method

作　　者：王志刚[1,2] 秦民坚[1,2] 郝利君[3] 孙洁[1,2] 

机构地区：[1]中国药科大学中药学院中药资源教研室,江苏南京211198 [2]中国药科大学教育部现代中药研究重点实验室,江苏南京211198 [3]中国药科大学基础部分析教研室,江苏南京211198

出　　处：《中国野生植物资源》2009年第5期54-57,共4页Chinese Wild Plant Resources

摘　　要：目的:对超声法提取菝葜中总黄酮的工艺进行优化。方法:利用超声法提取菝葜中的总黄酮,考察了超声时间、超声功率、乙醇浓度、固液比4个因素对总黄酮得率的影响,通过单因素试验和正交试验确定了提取的最佳工艺条件。结果:最佳提取工艺为超声功率250W、40KHz,超声时间30min,乙醇浓度80%,液固比30。Objective：The research aimed to optimize the extraction technology of total flavonoids from Smilax china L. by ultrasonic method. Methods： Total flavonoids was extracted from Smilax china L. by ultrasonic method. The effects of four factors including ultrasonic time, ultrasonic power, ethanol concentration and liquid - solid ratio on the yield of total flavonoids were investigated. The optimum technological conditions of total flavonoids exaction were confirmed through single factor test and the orthogonal test. Results： The optimum exaction technology conditions were as follows： ultrasonic power for 250W, 40KHz, ultrasonic time of 30 min, 80% ethanol concentration and solid-liquid ratio of 30.

关 键 词：菝葜 总黄酮 正交设计 超声提取 

分 类 号：R284.1[医药卫生—中药学]