鸭肠炎病毒UL41、UL42基因的克隆与分析  

Cloning and sequence analysis of duck enteritis virus UL41 and UL42 gene

作　　者：陈普成[1] 柳金雄[1] 曾青华[2] 姜永萍[1] 田国彬[1] 陈化兰[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所农业部动物流感重点实验室/兽医生物国家重点实验室,黑龙江哈尔滨150001 [2]湖南农业大学动物医学院,湖南长沙410128

出　　处：《中国预防兽医学报》2009年第12期988-990,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家科技支撑计划项目(2006BAD06A05)

摘　　要：依据GenBank登录的鸭肠炎病毒(DEV)核苷酸序列设计引物,利用长片段PCR技术扩增了DEV基因组UL36与UL43基因之间的未知序列,扩增所得片段长度约为15kb,经EcoRⅤ单酶切,将其中的3.9kb片段克隆到pUC18中。序列分析表明该3.9kbEcoRⅤ片段含有2个完整的转录方向相反的与单纯疱疹病毒(HSV)UL41和UL42基因同源的ORF,命名为DEV UL41和UL42基因。通过氨基酸序列比对发现:DEVUL41基因含有5个高度保守位点,而UL42含有2个,进化树分析表明DEV与疱疹病毒科α疱疹病毒亚科的马立克病毒、火鸡疱疹病毒的进化关系非常相近,为DEV的分类提供了参考依据。A DNA fragment more than 15.0 kb located between UL35 and UL43 in the duck enteritis virus （DEV） genome was amplified. A 3.9 kb EcoR V fragment of the PCR product was cloned and sequenced. Two major open reading frames homologous to the herpes simplex virus （HSV） UL41 and UL42 genes respectively were identified. The amino acid sequences of the two deduced DEV proteins displayed five highly conserved sites in the UL41 and two consensus regions in UL42 when compared with other twelve alphaherpesviruses. Phylogenetic analysis showed that DEV was closely related to avian herpes viruses of the alphaherpesviruses, which provided further proof for the classification of the virus.

关 键 词：DEV UK41基因 UIA2基因 进化分析 分类 

分 类 号：D852.65[政治法律—政治学]