海桐皮提取物的抗肿瘤活性及其机制研究  

Anti-tumor activity of Erythrina variegata L. extract and its mechanism of action

作　　者：张虹[1] 向俊锋[1] 谭莉[1] 戴玮[1] 白鸽[2] 刘岩[1] 曹学丽[2] 唐亚林[1] 

机构地区：[1]中国科学院化学研究所分子动态与稳态结构国家重点实验室,北京100190 [2]北京工商大学北京市植物资源研究开发重点实验室,北京100037

出　　处：《药学学报》2009年第12期1359-1363,共5页Acta Pharmaceutica Sinica

摘　　要：本研究探讨了海桐皮提取物的抗肿瘤活性及其作用机制。首先应用MTT法对海桐皮提取物进行体外细胞毒效应实验。为明确体内抗肿瘤疗效,建立了Lewis肺癌的小鼠模型,通过对比各组小鼠的相对肿瘤增值率、肿瘤生长曲线、抑瘤率、瘤重等指标考察海桐皮提取物的抑瘤效果。并通过G-四链体的稳定性实验,探讨了海桐皮的抗肿瘤作用机制。结果显示,海桐皮提取物在体外对肿瘤细胞增生具有抑制作用,在体内的抗肿瘤药效实验中也表现出明显的抗肿瘤活性。从G-四链体的稳定性实验结果可以看出,随着海桐皮提取物加入量的增加,G-四链体的熔点(Tm)逐步提高,其对G-四链体结构具有很好的稳定作用。The anti-tumor activities and mechanism of Erythrina variegata L. extract were investigated. Firstly, the MTT method was used to evaluate the inhibitory activity of the Erythrina variegata L. extract on proliferation of cancer cell lines. Moreover, in order to determine its anti-tumor effect in vivo, the Lewis lung cancer mice model was established. By comparing the relative tumor proliferation rates, growth curves, inhibition rates of different groups, the anti-tumor effect was evaluated. Furthermore, the anti-tumor mechanism of Erythrina variegata L. extract was studied by using G-quadruplex stability experiment. In the in vitro anti-liver cancer experiment, the Erythrina variegata L. extract has shown obvious anti-tumor effect on various tumor cells And in the in vivo experiment, it exhibited significant anti-tumor effect. Besides, from the result of G-quadruplex stability experiment, we can see that the quadruplex structure show increasing Tm values with increasing amounts of Erythrina variegata L. extract.

关 键 词：海桐皮 抗肿瘤活性 G-四链体 

分 类 号：R285[医药卫生—中药学]