金丝桃苷对鸭乙肝病毒cccDNA清除及免疫调节作用探讨  

Effects of hyperin on the cccDNA of duck hepatitis B virus and its immunological regulation

作　　者：耿淼[1] 王建华[1] 陈红艳[1] 杨新波[1] 黄正明[2] 

机构地区：[1]解放军总医院老年医学研究所,北京100853 [2]解放军302医院药学部,北京100039

出　　处：《药学学报》2009年第12期1440-1444,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30572350)

摘　　要：据世界卫生组织报道,全球约20亿人曾感染过乙肝病毒,乙型肝炎仍然严重威胁人类健康,其中很大一部分都会发展成为慢性肝炎、肝硬化甚至肝癌。目前常见的乙肝治疗药物主要以核苷（酸）类似物及干扰素为主,但由于存在疗程长、副作用严重及停药后反弹等因素,目前的治疗效果仍不理想。近年来的研究表明,机体特异性免疫功能低下,The aim of this study is to investigate the effect of hyperin on the cccDNA of duck hepatitis B virus and its immunological regulation. Duck hepatitis B virus （DHBV） infection model and normal mouse spleen lymphocyte were used to evaluate the anti-HBV and immunoregulation effects. The DHBV-DNA of serum was detected at different time points by using serum DOT-BLOT hybridization. Polymerase chain reaction （PCR） was used for the determination of nuclear covalent closed circular DNA （cccDNA）. Cytokine secretion was determined by ELISA method. DHBV-DNA were inhibited by hyperin （25 or 50 mg.kg^-1）, while cccDNA of liver could be eliminated efficiently by hyperin （25 or 50 mg.kg^-1, P〈0.05, P〈0.01）. The T helper 1 effector cytokine was markedly enhanced by hyperin （25 or 50 μg.mL^-1, P 〈 0.01）. In conclusion, hyperin has anti-HBV activity via multiple targets and pathways, and cccDNA may be one of the important targets.

关 键 词：金丝桃苷 乙肝病毒共价闭合DNA 免疫调节 

分 类 号：R285[医药卫生—中药学]