孕酮和米非司酮对蒙古绵羊输卵管上皮细胞β-防御素mRNA表达的影响  

Impact of Progesterone and Mifepristone on β-Defensin mRNA Expression in Epithelial Cells of the Fallopian Tubes in Sheep

作　　者：唐博[1,2] 杜晨光[2] 付本懂[1] 锡林高娃[2] 曹贵方[2] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]内蒙古农业大学动物科学与医学学院,呼和浩特010018

出　　处：《动物学杂志》2009年第6期151-155,共5页Chinese Journal of Zoology

基　　金：国家自然科学基金项目(No.30460093);内蒙古自治区自然科学基金项目(No.20080404MS0503)

摘　　要：β-防御素(β-defensin)在雌性动物生殖道广泛存在,起免疫防御作用。为了研究孕酮与雌性生殖道β-防御素mRNA表达的关系,本实验建立绵羊(Ovisaries)输卵管上皮细胞培养体系,添加不同浓度孕酮(10-6,10-7,10-8,10-9和10-10mol/L)和孕酮拮抗剂米非司酮后提取细胞总RNA,利用实时定量PCR测定β-防御素mRNA的相对表达量。结果显示,一定浓度的孕酮(10-6,10-7,10-8和10-9mol/L)对培养的输卵管上皮细胞β-defensin mRNA的表达有促进作用,且不同浓度的孕酮对β-defensinmRNA的表达的影响程度不同。米非司酮极显著抑制了孕酮诱导的β-defensin mRNA的表达。结果表明,孕酮通过与孕酮受体结合促进β-defensin mRNA的表达,推断雌性生理周期下孕酮可能通过作用于β-defensin等影响自身免疫。β-defensin is widely detected in female reproductive tracts and is regarded to play an important role in the immune defence.This study aimed to investigate the regulation of β-defensin mRNA expression by progesterone in female reproductive tract. The epithelial cells of the Fallopian tube of Mongolian sheep was cultured in vitro. Cells were treated with different concentrations of progesterone （10^ -6 ,10 ^-7 ,10^ -8 ,10^ -9 and 10 ^-10 mol/L） with or without mifepristone （the antagonist of progesterone）,total RNA was extracted from cells,and the relative expression of β-defensin mRNA was detected using real time RT-PCR.The results showed that different concentrations of progesterone variously enhanced the expression of β-defensin mRNA in the epithelial cells of the Fallopian tube,while mifepristone significantly inhibited the expression of β-defensin mRNA induced by progesterone.These results illuminate that progesterone promotes the expression of β-defensin and that progesterone may affect the autoimmunity of the female animals via β-defensin pathway.

关 键 词：Β-防御素 实时定量PCR 17-Β-雌二醇 米非司酮 

分 类 号：Q492[生物学—生理学] S852.1[农业科学—基础兽医学]