大黄素对模拟冷缺血再灌注后肝细胞内钙离子浓度及细胞凋亡的影响  

Effect of Emodin on intracellular calcium concentration([Ca^(2+)]i) and apoptosis of hepatic cells after simulated cold ischemia-reperfusion

作　　者：祁翔[1] 吕毅[1] 沈乃营[1] 刘昌[1] 刘学民[1] 王博[1] 

机构地区：[1]西安交通大学医学院第一附属医院肝胆外科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2009年第6期669-671,676,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的研究中药大黄素对模拟冷缺血再灌注后肝细胞内钙离子浓度及细胞凋亡的影响。方法体外培养肝细胞株HL-7702,随机分为对照组和大黄素处理组。对照组未予大黄素处理,大黄素处理组按100、10、1μmol/L分为高、中、低3个浓度组,建立模拟冷缺血再灌注模型,流式细胞技术检测各组细胞内钙离子浓度及细胞凋亡水平,分别检测各组细胞培养上清液乳酸脱氢酶水平。结果冷缺血8 h再灌注6 h后,高、中、低浓度大黄素处理组钙离子荧光强度分别为(24.12±0.51)(、26.35±1.34)、(39.12±1.94),均显著低于对照组的105.29±1.01(P<0.01)。高、中、低浓度大黄素处理组细胞凋亡率分别为(5.46±0.41)%、(10.64±0.64)%、(11.90±0.50)%,均显著低于对照组的(25.40±1.41)%(P<0.01)。高、中浓度大黄素处理组上清液LDH含量分别为(179.67±18.57)u/L(、198.83±14.22)u/L,显著低于对照组的(351.33±34.16)u/L(P<0.01)。结论大黄素可降低模拟冷缺血再灌注后的肝细胞内钙离子浓度,抑制细胞凋亡,减轻肝细胞损伤。Objective To investigate the effect of Emodin on intracellular calcium concentration （[Ca^2＋ ]i） and apoptosis of hepatic cells after simulated cold ischemia-reperfusion. Methods Glucose-oxygen deprivation, low temperature, subsequent reoxygenation and rewarming were used to induce ischemia-reperfusion injury model in cultured hepatic ceils which were divided into 4 groups： control group and Emodin-treated group（100, 10 and 1 μmol/L groups）. The intracellular calcium concentration （[Ca^2＋]i） and apoptosis rate were determined by flow cytometry （FCM） respectively; the content of lactate dehydrogenase （LDH） in supernatant was tested. Results IntraceUular calcium fluorescence intensity in Emodin-treated groups of high, medium and low density was 24.12±0.51, 26.35±1.34 and 39.12 ± 1.94, respectively, which were significantly lower than 105.29 ±1.01 in control group（P〈0.01）. Apoptosis rate in Emodin-treated groups of high, medium and low density was 0. 054 6±0. 004 1, 0. 106±0. 006 4 and 0. 119 0±0. 005 0, respectively, which were significantly lower than 0. 254 0±0. 014 1 in control group（P〈0.01）. LDH was （198.83±14.22）u/L and （179.67± 18.57）u/L in Emodin-treated groups of medium and high density respectively, which were significantly lower than （351.33 ±34.16）u/L in control group （P〈0.01）. Conclusion Emodin could reduce [Ca^2＋ ]i and inhibit apoptosis of hepatic cells after simulated cold ischemia-reperfusion, thus protecting hepatic cells effectively.

关 键 词：大黄素 肝细胞 冷缺血再灌注 细胞内钙离子浓度 凋亡 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学] R657.3[医药卫生—基础医学]