假型逆转录病毒载体高效介导兔平滑肌细胞基因转染  

High-efficiency gene transfer into rabbit smooth muscle cells by pseudotyped retroviral vector

作　　者：裴斐[1] 何蕊[1] 李俊彦[1] 余红[1] 

机构地区：[1]西安交通大学医学院第二附属医院心胸外科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2009年第6期766-768,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30470456)~~

摘　　要：目的构建假型逆转录病毒载体MuLV／VSV-G，并用于转染兔平滑肌细胞，为兔平滑肌细胞基因转染寻找一种高效的载体。方法构建含有报道基因lacZ的假型逆转录病毒载体MuLV／VSV—G，测定滴度，并转染兔平滑肌细胞，观察其转导效率。并与MuLV的转导效率进行比较。结果构建的MuLV／VSV-G载体，病毒滴度为6～7．8×10^6CFU，转染兔平滑肌细胞的效率是（92±12）％。而MuLV的转导效率为（24土5）％。结论成功构建了假型逆转录病毒载体MuLV／VSV-G载体，该载体作为一种高效的载体可用于兔平滑肌细胞基因转染。Objective To construct pseudotyped retroviral vector MuLV/VSV-G and transfer it into rabbit smooth muscle cells （SMC） in order to provide a high-efficiency vector for SMC gene transfer. Methods We constructed pseudotyped retroviral vector MuLV/VSV-G containing the previously reported gene lacZ, determined the titer, and determined the efficiency of gene transfer into SMC mediated by pseudotyped retroviral vector MuLV/VSV-G. Finally the transfer efficiency was compared with that by MuLV. Results MuLV/VSV-G vector was constructed. The titer of the vector was 6- 7.8 ×10^6CFU, the transfer efficiency was （92 ±12）% by using MuLV/VSV-G vector and （24±5）% by MuLV vector. Conclusion Pseudotyped retroviral vector MuLV/VSV-G which was constructed successfully is a kind of high-efficiency gene transfer vector in smooth muscle cells.

关 键 词：假型逆转录病毒载体 平滑肌细胞 转染 

分 类 号：R373[医药卫生—病原生物学]