磁性脂质超声微泡造影剂的制备及影像增强的实验研究  

Experimental study on preparation and image contrast enhance of magnetic lipid ultrasound micro-bubble

作　　者：李华梅[1] 张佳[1] 张东生[1] 

机构地区：[1]东南大学基础医学院病理学与病理生理学系,江苏南京210009

出　　处：《东南大学学报（医学版）》2009年第6期474-478,共5页Journal of Southeast University(Medical Science Edition)

基　　金：国家863计划项目(2007AA03Z356);国家自然科学基金资助项目(30770584)

摘　　要：目的：探讨自制磁性脂质微泡超声造影剂在体外的基本特性及体内的影像增强效果，为研制既能在超声诊断中使影像增强，又能对肿瘤进行热疗的超声造影剂提供理论基础和实验依据。方法：（1）采用化学共沉淀法制备Fe3O4磁性纳米材料，机械振荡法制备磁性脂质微泡。（2）马尔文粒径测量仪测量磁性脂质微泡粒径，血球计数板测量浓度，扫描电镜及能谱分析对其形态和成分进行分析。（3）将已制备的磁性脂质微泡原溶液放入4℃冰箱里储存2周后观察其形态和粒径的变化。（4）大型彩超仪评价其在兔肝脏的显像效果。（5）分别将含有不同浓度磁性纳米粒子的脂质微泡置于频率230kHz、输出电流30A的SPG-06A高频磁感应加热设备平板线圈上加热1h，观察其体外升温情况。结果：此微泡平均粒径为1127nm，浓度为3×10^9ml^-1。在4℃冰箱中放置2周后，其形态和粒径未发生明显改变。经耳缘静脉注入兔肝脏后，肝实质回声可见明显增强。一定浓度的磁性脂质微泡在一定磁场作用下具有升温恒温的特性，温度可恒定在42～62℃之间。结论：此磁性脂质微泡造影剂粒径小，性质稳定，影像增强效果好，在磁场作用下具有升温恒温能力，为今后，临床的进一步应用提供了实验依据。Objective To study the characteristics of self-made magnetic lipid micro-bubble ultrasound contrast agent in vitro and image contrast enhancement in vivo and provide the evidence of theory and experiment for making the ultrasound micro-bubble providing image enhancement and hyperthermia to the tumor. Methods （ 1 ） Fe3 O4 magnetic nano-particles and magnetic lipid micro-bubble were prepared by chemical co-precipitation technique and mechanical vibration technique. （2） The surface morphology, size distribution and concentration of the contrast agent were measured by Malvern, at the same time the appearance and composition was analyzed by SEM and EDS. （3） Morphology and size of the lipid micro-bubble saved in refrigerator at 4 ℃ were observed after two weeks. （4） Rabbits were examined to observe the enhancement time and intensity in liver using color ultrasound. （5） Thermodynamic test was used to observe temperature change of various doses of Fe3O4 magnetic lipid micro-bubble under alternating magnetic field （AMF）. Results The self-made magnetic lipid micro-bubble were well shaped,with a mean diameter of 1 127 nm and at a concentration of 3×10^9 ml^-1. The morphology and size of the lipid micro-bubble in refrigerator with 4 ℃ were consistent with the previous ones. The prepared Fe3 04 magnetic lipid micro-bubble raised the temperature and maintained it at 42-62 ℃ in certain magnetic field. Conclusion The magnetic lipid micro-bubble steadily and conspicuously functions as a contrast enhancer that provide dependable guarantee for clinic application in future.

关 键 词：脂质微泡 磁性纳米粒子 影像增强 

分 类 号：R-33[医药卫生] R454