甘草甜素类药对化学损伤人肝细胞的保护作用比较  

Comparison of protective effect of glycyrrhizin drugs on human hepatocytes injured by D-galactosamine and carbon tetrachloride

作　　者：张群[1] 张丽峰[2] 陈鹏[3] 孙华丽[3] 张建军[3] 

机构地区：[1]东南大学附属中大医院感染病科,南京210009 [2]成都中医药大学附属医院血管外科,成都610072 [3]泸州医学院附属医院感染病科,泸州646000

出　　处：《重庆医科大学学报》2009年第11期1491-1494,共4页Journal of Chongqing Medical University

摘　　要：目的:研究4种(3类)甘草甜素药物对半乳糖胺(D-GalN)和四氯化碳(CCl4)损伤培养人肝细胞(L-02)的保护作用及差异,筛选出疗效好的药物,指导临床应用。方法:培养L-02,用复方甘草酸单铵(Compound ammonium glycyrrhizin,CAG)、复方甘草酸苷(Compound glycyrrhizin,CG)、甘草酸二铵(Diammonium glycyrrihizinate,DG)和异甘草酸镁(Magnesium isoglycyrr hizinate,MI)分别进行保护,再经D-GalN或CCl4处理。观察肝细胞生长状态、测定AST及LDH酶活力、测定细胞内谷胱甘肽(GSH)含量。从而评价CAG、CG、DG和MI对D-GalN和CCl4损伤L-02细胞的保护作用。结果:浓度为1 mg/ml时,CAG、CG、DG和MI均能显著抑制D-GalN和CCl4所致的AST及LDH释放,提高细胞的存活率。其中CAG抑制D-GalN所致的AST效果显著优于CG、DG和MI(P<0.05);CAG抑制CCl4所致的AST及LDH释放的效果显著好于DG和MI;CG抑制D-GalN所致的AST和LDH释放效果显著(P<0.05)好于MI。浓度为1 mg/ml的CAG、CG、DG和MI 4种药物均能显著抑制2种化学损伤细胞内的GSH降低,其中CAG效果最明显(P<0.05)。结论:1 mg/ml的CAG、CG、DG和MI 4种药物对D-GalN和CCl4致人肝细胞损伤均有保护作用,其机制可能与抑制GSH降低相关。4种甘草甜素药物,CAG保护肝细胞效果最为显著,CG、DG和MI作用依次减低。Ojective： To investigate the effects and the differences of the four （3 categories） glycyrrhizin drugs in protecting human hepatocytes （L-02） from the injury of galactosamine （D-GaIN） and carbon tetraehloride （CCl4）, and to screen a better one for clinical application. Methods ：The cultured L-02 were pre-treated with compound ammonium glycyrrhizin（CAG）, compound glycyrrhizin（CG）, diammonium glycyrrihizinate （DG） and magnesium isoglyeyrrhizinate （MI）, respectively, for protecting these cells from the injury made with CC14 or D-GaIN. Then, the liver cell growth status were observed, and the AST and LDH enzyme activity in the cultural supernatant were determined, and cellular glutathione （GSH） content in the cultural supernatant were tested. All the values above were analyzed to compare the protective effects of the four glycyrrhizin drugs. Results：At the concentration of lmg/ml, the four glycyrrhizin drugs could improve the survival rate of cells, and significantly inhibit release of AST and LDH induced by D-GaIN or CC14. Moreover, the inhibition effect of CAG on AST release induced by D-GaIN was significantly（P〈0.05 ） better than that of CG, DG and MI, and the inhibition effects of CAG on AST and LDH release induced by CC14 were significantly（P〈0.05 ） better than those of CG, DG and MI. The inhibition effects of CG on AST and LDH release induced by D-GaiN were significantly（P〈0.05 ） better than that of MI. At the Concentration of 1 mg/ml, the four glycyrrhizin drugs could increase the decreased cell mitochondriai membrane potential caused by D-GaiN or CC14. These four glyeyrrhizin drugs could significantly inhibit the intraeellular GSH abatement induced by D-GaIN or CC14. Of which, CAG had the most significant effect（P〈0.05 ）, and CG was better than MI（P〈0.05 ）. Conclusions：At the concentration of 1 mg/ml, the four glycyrrhizin drugs have protective effects on the D-GaiN or CC14 injured human hepatoeytes. The mechanism of these protective ef

关 键 词：半乳糖胺 四氯化碳 甘草甜素 肝细胞 药物作用 

分 类 号：R965.1[医药卫生—药理学]