测定GITR和GITRLELISA的建立及其临床初步应用  

The establishment of an ELISA for measuring GITR and GITRL and its clinical application

作　　者：孙立山[1] 范列英[1] 张慧[1] 郑慧雅[1] 宗明[1] 朱海波[1] 

机构地区：[1]同济大学附属上海市东方医院,上海200120

出　　处：《检验医学》2009年第12期891-894,共4页Laboratory Medicine

基　　金：上海市自然科学基金项目(05ZR14099);浦东新区科技发展基金创新攻关项目(PKJ-2006-Y16)

摘　　要：目的建立测定糖皮质激素诱导的肿瘤坏死因子受体(GITR)及其配体(GITRL)的定量酶联免疫吸附试验(ELISA),并探讨GITR及GITRL定量测定在类风湿性关节炎(RA)及肿瘤疾病的诊断或病情监测中的临床应用。方法以鼠抗人GITR、GITRL单克隆抗体为包被抗体,以生物素-辣根过氧化物酶标记链霉亲和素(SA-HRP)检测系统,建立GITR和GITRL定量检测的双抗体夹心ELISA。测定105例RA患者、54例肿瘤患者及100名健康体检者的血清GITR、GITRL含量,并探讨其与疾病的关系。结果建立的测定GITR、GITRL定量ELISA线性相关系数的平方(r2)分别为0.94和0.90。RA组血清中GITR、GITRL含量均明显高于正常对照组(P均<0.001);肿瘤组GITR高于正常对照组(P<0.001),GITRL与正常对照组差异无统计学意义(P>0.05)。结论本研究成功建立了GITR、GITRL定量ELISA,血清GITR、GITRL含量测定可能会成为RA新的辅助性诊断指标和病情监测指标。Objective To establish an enzyme-linked immunosorbent assay（ELISA） for measuring glucocorticoid-induced tumor necrosis factor receptor（GITR）and glucocorticoid-induced tumor necrosis factor receptor ligand（GITRL）,and explore the clinical application of the measurement of GITR and GITRL in the diagnosis and monitoring rheumatoid arthritis（RA） and tumor diseases.Methods With mouse monocolonal anti-human GITR and GITRL antibody as coating antibody and biotin-streptavidin-horseradish peroxidase（SA-HRP） as detecting system,a double antibodies sandwich ELISA was established for measuring GITR and GITRL.The serum levels of GITR and GITRL from 105 patients with RA,54 patients with tumor and 100 healthy subjects were measured and the relationship of GITR and GITRL with RA and tumor diseases was analyzed.Results The correlation coefficient（r^ 2） was 0.94 for GITR assay and 0.90 for GITRL assay.The levels of GITR and GITRL in patients with RA were higher than those in healthy subjects（P〈0.001）.The levels of GITR in patients with tumor were higher than those in healthy subjects（P〈0.001）,and there was no significant difference for GITRL levels（P〉0.05）.Conclusions An ELISA for measuring the GITR and GITRL in serum is successfully established.GITR and GITRL in serum may be new assistant diagnostic or monitoring parameters for RA.

关 键 词：糖皮质激素诱导的肿瘤坏死因子受体 糖皮质激素诱导的肿瘤坏死因子受体配体 酶联免疫吸附试验 类风湿性关节炎 

分 类 号：R446.61[医药卫生—诊断学]