嗜盐古菌ABDH12的部分生理生化特征及Br蛋白基因部分序列的研究  

Studies on Physiological and Biochemical Characters and Bacteriorhodopsin Gene of Strain ABDH12 of XingjiangAibi Lake

作　　者：旭格拉.哈布丁 陈瑾[1] 迪丽乌孜.艾尼瓦尔 黄瑞虎[1] 周宇光[3] 迪丽拜尔.托乎提 

机构地区：[1]新疆师范大学生命与环境科学学院,新疆乌鲁木齐830054 [2]新疆大学生命科学与技术学院,新疆乌鲁木齐830046 [3]中国科学院微生物研究所,北京100080

出　　处：《新疆师范大学学报（自然科学版）》2009年第4期78-83,共6页Journal of Xinjiang Normal University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(30760003);科技部中国科学院微生物研究所资源平台项目;新疆师范大学校级重点实验室资助项目;新疆师范大学优秀青年教师科研启动基金(XJNU0734)

摘　　要：文章分析新疆艾比胡嗜盐古生菌物种与细菌视紫红质(bacteriorhodopsin,BR)蛋白基因资源,对分离纯化到的极端嗜盐古生菌菌株ABDH12,对其进行了生理生化特性研究,采用PCR方法扩增技术和编码螺旋C至螺旋G的BR蛋白基因片断,测定了BR蛋白基因核心序列。基于生理生化特性和BR蛋白基因序列的同源性比较,以及系统发育分析表明,BR蛋白中对于完成质子泵功能以及与视黄醛结合的关键性氨基酸残基均为保守序列,位于膜内侧的序列比位于膜外侧的序列更保守,并且ABDH12中的螺旋C至螺旋G的蛋白与其他菌株差异明显,是两种新的BR蛋白,序列号为:FJ796897。迄今为止,国内极少有关艾比湖嗜盐古菌的BR蛋白研究的报道,文章可为今后研究同类极端环境中新的物种资源提供素材和参考。In order to study the halophiles and bacterio--op sin protein （bop） gene resource from AiBi Lake located in Xinjiang Region, ABDH12 from the isolates were picked out, and the study of physiological and biochemical characters, the genes encoding for bacterio--op sin protein fragment were proliferated by PCR. Their nucleotide sequences were determined subsequently. On the basis of phylogenetic analysis of the bop gene sequences, It is indicated that the bop genes of halophilic archaea from AiBi lake have some diversity and terrain traits, homology alignment using the deduced partial amino acid sequence of ABDH12 Br protein with other Br proteins showed that these Br protein are obviosly different to others. These facts indicated that the two Br in halophilic archaeum ABDH12 are a new Br protein .

关 键 词：艾比胡 嗜盐古生菌 细菌视紫红质蛋白 

分 类 号：Q933.1[生物学—微生物学]