马桑叶中黄酮类化合物的生物活性研究  

Physiological Activity of Flavonols Isolated from the Leaves of Coriaria sinica Maxim

作　　者：张雁冰[1] 戴桂馥[2] 侯立芬[3] 袁嘉琪[4] 张赛扬[1] 刘宏民[1] 

机构地区：[1]郑州大学药学院,郑州450001 [2]郑州大学生物工程系,郑州450001 [3]郑州大学化学系,郑州450001 [4]武汉大学化学与分子科学学院,武汉430072

出　　处：《郑州大学学报（理学版）》2009年第4期62-66,共5页Journal of Zhengzhou University:Natural Science Edition

摘　　要：探讨马桑叶中6种黄酮类化合物的体外抗氧化作用及对小鼠脾淋巴细胞增殖的影响,通过测定其体外抑制红细胞溶血时间研究其抗氧化性.采用MTT法,测定了协同ConA对小鼠T淋巴细胞增殖的抑制作用,并测定了协同LPS对小鼠B淋巴细胞增殖的抑制作用.结果表明,这6种黄酮类化合物对AAPH诱导红细胞溶血有很好的抑制作用,其中效果最好的是槲皮素,槲皮素的糖苷强于山奈酚的糖苷;能协同ConA对小鼠T淋巴细胞增殖产生抑制作用,效果最好的是山奈酚-3-O-β-D-半乳糖(K-Gal)和山奈酚-3-O-β-D-葡萄糖(K-Glu);能协同LPS对小鼠B淋巴细胞增殖产生抑制作用,效果最好的是槲皮素-3-O--αL-阿拉伯糖(Q-Ara)和槲皮素(Q).The anti-oxidative activity of flavonols isolated from the leaves of Coriaria sinica Maxim and the effects on proliferation of mice spleen lymphocytes are studied.2,2 -azobis（2-amidinopropane）dihydrochloride（AAPH） induced oxidative hemolysis are used to study the effect of the six flavonols.The experiments inhibiting ConA to induce the proliferation reaction of T lymphocyte and inhibiting LPS to induce the proliferation reaction of B lymphocyte are done by MTT colorimetry.All the compounds show good inhibition effects on AAPH-induced hemolysis of human RBC. The effect of quercetin is the best. Quercetin glycosides are better than kaempferol glycosides. ConA to induce the proliferation reaction of T lymphocyte and LPS to induce the proliferation reaction of B lymphocyte can be inhibited by these compounds. Kaempferol-3-O-β-D-galactoside and kaempferol-a-O-β-D-glucoside show the best effects on the proliferation of T lymphocyte. Quercetin-3-O-β-L-arabinoside and quercetin show the best effects on the proliferation of B lymphocyte.

关 键 词：马桑 黄酮 抗氧化作用 淋巴细胞增殖 MTT比色法 

分 类 号：R932[医药卫生—生药学]