双酚A暴露对斑马鱼胚胎期代谢作用的影响  

Effects of bisphenol A on the metabolites of zebrafish embryos

作　　者：端正花[1,2] 朱琳[1] 赵娜[1] 卜文俊[2] Lam Siew Hong Gong Zhiyuan 邹莉[4] 李巍[4] Ong Eng Shi Ong Choon Nam 

机构地区：[1]南开大学环境科学与工程学院,环境污染过程与基准教育部重点实验室,天津300071 [2]南开大学生命科学学院,天津300071 [3]新加坡国立大学生物科学系,新加坡117543 [4]新加坡国立大学流行病学与公共卫生系,新加坡117543

出　　处：《中国环境科学》2009年第12期1318-1322,共5页China Environmental Science

基　　金：国家自然科学基金资助项目(30470319)

摘　　要：运用基于GC/MS的代谢组学方法,以受精卵在双酚A(BPA)中暴露4d后的斑马鱼胚胎为对象,研究了其体内挥发性和半挥发性代谢组分变化.暴露浓度设定为0,0.5,1.5,4.5mg/L,包括USEPA假定的"安全水平"(1.5mg/L).结果表明,1.5mg/LBPA暴露能使斑马鱼体内花生四烯酸和胆固醇的相对百分含量极显著降低,饱和脂肪酸、氨基酸、葡萄糖和肌糖的相对百分含量显著升高.因此,1.5mg/LBPA能干扰斑马鱼胚胎期的正常代谢.Metabonomics approach based on the GC-MS technique was used to characterize the perturbation of volatile and semi-volatile components in zebrafish embryos induced by bisphenol A （BPA） for 4 d since fertilization. The exposure concentrations of BPA were 0, 0.5, 1.5 and 4.5mg/L, including assumed＂safe level＂（1.5mg/L） that stated by US EPA. The relative proportions of arachidonic acid and cholesterol were decreased dramatically, and those of saturated fatty acid （SAF）, amino acid, glucose and inositol were increased significantly. Therefore, 1.5 mg/L BPA would disturb the regular metabolizing of zebrafish embryos.

关 键 词：代谢组学 双酚A 斑马鱼 GC/MS 

分 类 号：X503.225[环境科学与工程—环境工程]