HPLC法同时测定元胡止痛分散片中延胡索乙素和欧前胡素的含量  

Determination of dl-tetrahydropalmatine and imperatorin in Yuanhuzhitong dispersible tablet by HPLC

作　　者：章军[1] 王跃生[1] 李慧[1] 张保献[1] 臧琛[1] 王国华[1] 欧阳旭[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2009年第12期10-12,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2006BAI09B08-06)

摘　　要：目的:建立简便、快速的同时测定元胡止痛分散片中延胡索乙素和欧前胡素的高效液相色谱分析方法。方法:甲醇回流提取延胡索乙素和欧前胡素,以Kromasil C18(5μm,4.6 mm×250 mm)为分析柱,以乙腈-甲醇-0.1%磷酸水溶液(三乙胺调pH=6.0)(41∶13∶46)为流动相;检测波长为280 nm;柱温40℃;流速为0.8 mL.min-1。结果:延胡索乙素峰和欧前胡素峰分离良好,阴性无干扰,方法学考察合格。结论:该方法适用于元胡止痛分散片中延胡索乙素和欧前胡素的含量测定。Objective：To develop a rapid HPLC method for determination of dl-tetrahydropalmatine and imperatorin in Yuanhuzhitong dispersible tablet.Method：dl-tetrahydropalmatine and imperatorin were extracted by reflux extraction method with methanol.A kromasil C_（18）（5 μm,4.6 mm×250 mm）column was used with the mobile phase of acetonitrile-methanol-0.1% phosphoric acid（pH was adjusted to 6.0 by triethylamine）（41∶13∶46）at the detection wavelength of 280 nm.The flow rate was 0.8 mL·min^（-1） at the column temperature of 40 ℃.Results：The dl-tetrahydropalmatine peak and imperatorin peak can be separated perfectly.The negative samples were not found interference in this condition.The method is accurate.Conclusion：The method is suitable for the determination of dl-tetrahydropalmatine and imperatorin in Yuanhuzhitong dispersible tablet.

关 键 词：元胡止痛分散片 延胡索乙素 欧前胡素 含量测定 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]