舒肝凉血方与阿那曲唑联用对去卵巢大鼠骨代谢的影响  

Effects of Shugan-liangxue Prescription Combined with Anastrozole on Bone Metabolism of Ovariectomized Rats

作　　者：刘素香[1] 任艳[1] 庄桂宝 李萍萍[1] 

机构地区：[1]北京大学,临床肿瘤学院、北京肿瘤医院暨北京市肿瘤防治研究所中西医结合科,恶性肿瘤发病机制及转化研究教育部重点实验室,北京100142

出　　处：《中国实验方剂学杂志》2009年第12期57-61,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京市中医局科技项目(2004京中重IV15)

摘　　要：目的:观察中药舒肝凉血方与芳香化酶抑制剂阿那曲唑联合使用对去卵巢SD大鼠血清雌二醇和骨代谢生化指标的影响。方法:采用去势法建立大鼠绝经后动物模型,随机将大鼠分为6组,即模型组、舒肝凉血方组(简称中药组)、阿那曲唑组、舒肝凉血方和阿那曲唑联合组(简称联合组)、阿仑磷酸钠组和假手术组。灌胃给药16周,双能X线骨密度仪测定大鼠腰椎和股骨的骨密度,放射免疫法检测血清雌二醇(E2)、骨钙素(BGP)和前列腺素E2(PGE2)的水平,ELISA法检测血清骨源性碱性磷酸酶(BAP)、Ⅰ型前胶原羧基端前肽(PICP)、吡啶诺林(PYD)、抗酒石酸盐酸性磷酸酶(TRAP)和Ⅰ型胶原交联羧基末端肽(ICTP)的水平。结果:中药组、阿那曲唑组和联合组的血清E2水平均明显低于模型组(P<0.05,P<0.01,P<0.001),骨密度与模型组没有明显差异。与阿那曲唑组相比,联合组能明显提高血清BGP的水平(P<0.05),显著降低PYD的水平(P<0.05)和PGE2的水平(P<0.01)。中药组和阿仑磷酸钠组均能明显降低PYD的水平,与模型组相比有显著差异(P<0.001,P<0.001)。结论:实验提示舒肝凉血方联合阿那曲唑在降低血清雌二醇的同时,对阿那曲唑导致的骨骼副作用有一定缓解作用。Objective：To investigate the effects of Shugan-liangxue prescription（SGLX） from traditional Chinese medicine combined with anastrozole（ANA） on the serum estradiol（E2） level and bone metabolism in ovariectomized（OVX） Sprague-Dawley（SD） rats.Methods：The postmenopausal animal model was established after OVX and testified by vaginal smear method.Six-month SD rats were randomly divided into model,SGLX,ANA,ANA＋SGLX,alendronate sodium（AL） and sham groups.After 16 weeks intragastric administration,the bone mineral density（BMD） of lumbar vertebra and femur were measured by dual energy X-ray absorptiometry.The serum E2,bone gla protein（BGP） and prostaglandin E2（PGE2） were detected by radioimmunoassay and bone alkaline phosphatase（BAP）,carboxyterminal propeptide of type Ⅰprocollagen（PICP）,pyridinoline（PYD）,tartrate-resistant acid phosphatase（TRAP）and cross-linked carboxyterminal telopeptide of typeⅠcollagen（ICTP） were determined by ELISA.Results： Serum E2 level decreased in SGLX,ANA and ANA＋ SGLX groups compared with the model group（P〈0.05,P〈0.01,P〈0.001）,BMD had no significant difference compared with the model.In ANA＋SGLX group,serum BGP level increased,PYD and PGE2 declined significantly compared with ANA group（P〈0.05,P〈0.05,P〈0.01）.PYD decreased greatly in SGLX and AL groups compared with the model（P〈0.001,P〈0.001）.Conclusions： This study showed that SGLX combined with ANA decreased the serum E2 level,meanwhile had the effect to relieve the side reaction of bone induced by ANA.

关 键 词：舒肝凉血方 阿那曲唑 骨密度 骨代谢 

分 类 号：R285.5[医药卫生—中药学]