超高效液相色谱法测定天然提取物中的黄樟素  

Determination of Safrole in Natural Extracts by UPLC

作　　者：王海利[1,2] 孟昭宇 汤建国 陆舍铭 朱瑞芝 刘正聪[3] 刘春波[2] 

机构地区：[1]红塔烟草集团有限责任公司技术中心,玉溪653100 [2]云南民族大学化学与生物技术学院,昆明650031 [3]云南大学化学科学与工程学院,昆明650091

出　　处：《香料香精化妆品》2009年第6期11-13,共3页Flavour Fragrance Cosmetics

摘　　要：建立了利用超高效液相色谱仪检测天然提取物中黄樟素含量的方法。以无水乙醇为溶剂,振荡萃取黄樟素后,直接进样。经配置FLRD检测器的超高效液相色谱于激发波长288nm、发射波长325nm下对其进行检测。所得回归方程为:Y=5.51×106X+3.48×105,(R2=0.9986,n=5),平均回收率为95.1%,检测限为38.3ng/g。该方法简便、准确,适用于对天然提取物中黄樟素的测定。A method is established for determination of safrole in natural extracts. The safrole in sample was extracted with solvent of anhydrous ethanol under shaking, and detected by UPLC with FLRD, the λEx was set at 288 nm and the λEra was set at 325nm. The regression equation： Y =5.51×10^6X＋3.48×10^5,（R^2=0.9986,n=5）. The average recovery of Safrole was 95.1%,The limit of detection was 38.3ng/g. This method is simple, quick and accurate for the determination of safrole in natural extracts.

关 键 词：黄樟素 香精 超高效液相色谱 

分 类 号：TQ658[化学工程—精细化工]