微波辅助双水相提取盾叶薯蓣中的皂苷成分  

Microwave-assisted Aqueous Two-phase Extraction of Steroidal Saponins in Dioscorea zingiberensis

作　　者：刘琳[1] 董悦生[1] 修志龙[1] 

机构地区：[1]大连理工大学环境生命学院生物科学与工程系,辽宁大连116024

出　　处：《过程工程学报》2009年第6期1147-1152,共6页The Chinese Journal of Process Engineering

摘　　要：采用微波辅助乙醇/硫酸铵双水相体系,从盾叶薯蓣中提取皂苷成分.实验结果表明,各种皂苷成分可有效地富集于醇相,盾叶新苷、三角叶皂苷、薯蓣皂苷元-葡萄糖三糖苷、薯蓣皂苷元-葡萄糖二糖苷、延龄草次苷的最大分配系数分别达89,91,93,117和153.微波辅助双水相提取皂苷成分的最佳药材/乙醇质量比为1:21,最佳相组成为乙醇25%(ω),硫酸铵19%(ω),该条件下的提取率为,总皂苷95.1%,盾叶新苷99.8%,三角叶皂苷97.4%,薯蓣皂苷元-葡萄糖三糖苷97.3%,薯蓣皂苷元-葡萄糖二糖苷86.6%,延龄草次苷63.2%.Microwave-assisted aqueous two-phase extraction of steroidal saponins in Dioscorea zingiberensis was studied by ethanol/ammonium sulphate system. The results showed that almost all the five main saponins were enriched in the top phase, and the maximum partition coefficients of Zingibernsis newsaponin, deltonin, Diosgenin-triglucoside, Diosgenin-diglucoside and trillin were 89, 91, 93, 117 and 153, respectively. The optimum mass ratio of material to ethanol was 1：21. The optimum system was composed of 25%（ω） ethanol and 19%（ω） （NH4）：SO4. Under the optimum condition, the yield of total saponins was 95.1%, and the yields of Zingibernsis newsaponin, deltonin, Diosgenin-triglucoside, Diosgenin-diglucoside and trillin were 99.8%, 97.4%, 97.3%, 86.6% and 63.2%, respectively.

关 键 词：微波辅助双水相萃取 乙醇/硫酸铵体系 盾叶薯蓣 甾体皂苷 提取率 

分 类 号：TQ460.6[化学工程—制药化工]