实验性糖尿病大鼠坐骨神经TNF-α表达的动态变化  

Dynamic TNF-α expression of sciatic nerve in diabetes mellitus rats

作　　者：杨薇[1] 吴江[1] 朱丹[1] 白晶[1] 

机构地区：[1]吉林大学第一医院神经内科,吉林长春130021

出　　处：《中风与神经疾病杂志》2009年第6期649-652,共4页Journal of Apoplexy and Nervous Diseases

基　　金：吉林省科技厅项目(200705170)

摘　　要：目的应用免疫组织化学的方法研究实验性糖尿病大鼠(DM大鼠)坐骨神经肿瘤坏死因子(tumor necrosis factor-α,TNF-α)的表达变化的动态规律。方法采用大鼠腹腔内注射链脲菌素(STZ)溶液,制备实验性糖尿病大鼠模型。将80只Wistar大鼠随机分为正常对照组(NC组)、成模即刻(0周)组、成模后4周组、8周组、12周组。应用免疫组织化学的方法观察TNF-α在大鼠下肢坐骨神经中的动态表达规律。结果DM各组TNF-α棕黄色异染颗粒在坐骨神经轴索中的表达逐渐减少,与NC组有极显著差异(P<0.01)。其中,DM8周组坐骨神经轴索中TNF-α棕黄色异染颗粒减少最明显,分别与DM4周、DM12周组之间有极显著差异(P<0.01)。DM各组坐骨神经髓鞘中TNF-α棕黄色异染颗粒逐渐增加,与NC组有极显著差异(P<0.01);DM4周组、8周组、12周组之间相比具有极显著差异(P<0.01)。结论正常生理情况下,TNF-α在大鼠坐骨神经中呈现轴索强阳性表达,髓鞘阴性表达。在糖尿病状态时,TNF-α在轴索的表达逐渐减少,8周时达到高峰。TNF-α在髓鞘的表达随着病程的延长而逐渐增加,这可能是糖尿病的发病机制之一。Objective To observe the dynamic expression of tumor necrosis factor-α（TNF-α）in the diabetes mellitus（DM） rats＇sciatic nerve by immunohistochemistry methods.Methods Streptozocin（STZ）（60mg/kg body weight） intraperitoneal injection was applied to make experimental DM rat models.80 Wistar rats were randomly divided into normal control group（NC group）,DM 0w group,DM 4w group,DM 8w group,DM 12w group.Dynamic expressions of TNF-α were observed in the DM rats＇ sciatic nerve by immunohistochemical methods.Results Since 4w after STZ injection,TNF-α expression in DM group rats＇ sciatic nerve axon gradually decreased with a peak at 8w.Compared with the NC group,DM 4w,DM8w and DM12w group rats showed significant differences in TNF-α expressions of sciatic nerve axon（P〈0.01）.Compared with DM 8w group,TNF-α expressions of DM 4w and DM 12w group showed significant differences respectively（P〈0.01）.Meanwhile,since 4w after STZ injection,TNF-α expression in DM group rats＇ sciatic nerve myelin sheath gradually increased.Compared with the NC group,DM 4w,DM8w and DM12w group rats showed significant differences in TNF-α expressions of sciatic nerve myelin sheath（P〈0.01）.Significant differences were observed among DM 4w,DM 8w or DM 12w group respectively（P〈0.01）.Conclusion Under normal physiological state,positive TNF-α expression is observed in sciatic nerve axon and negative expression in myelin sheath of DM rats＇ sciatic nerve.TNF-α expression in axon gradually decreases with a peak at 8w and increases in myelin sheath aggravating with the extension of DM course.It may be a possible mechanism during DM development.

关 键 词：实验性糖尿病大鼠 坐骨神经 TNF-Α 免疫组织化学 

分 类 号：R587.1[医药卫生—内分泌] R745.42[医药卫生—内科学]